利多卡因对兔内毒素血症肺细胞膜磷脂酶A_2及膜流动性的影响

Effects of lidocaine on phospholipase A_2 and fluidity of lung cell membrane during rabbits endotoxemia

目的:探讨利多卡因对兔内毒素血症肺细胞膜磷脂酶A_2(PLA_2)及膜流动性的影响。方法:24只日本大耳白兔随机分为对照组、内毒素(ET)致病组和利多卡因预处理组,每组8只。分别用微量滴定法、高效液相色谱法和DPH法测定肺和红细胞膜PLA_2活性,肺细胞膜磷脂PS、PE、PC及肺和红细胞膜脂流动性。结果:利多卡因预处理组肺细胞膜及血浆PLA_2活性水平显著低于ET致病组,肺细胞膜磷脂PS、PE、PC显著高于ET致病组,肺和红细胞膜流动性亦显著高于ET致病组。结论:利多卡因预处理能显著抑制肺细胞膜和血浆PLA_2活性,减少肺细胞膜磷脂降解,增加肺和红细胞膜流动性,稳定细胞膜功能。

To investigate the effects of lidocaine on phospholipase A_2(PLA_2) and fluidity of lung cell membrane during rabbits endotoxemia. Method: Twenty-four white rabbits were randomly assigned to receiving one of three treatmemnts (n=8 for each group): infusion of saline(control group), infusion of Escbericbia coli endotoxin(endotoxin group), infusion of endotoxin with lidocaine pretreatment (lidocaine group). The PLA_2 activity, PS, PE and PC contents of lung cell membrane fluidity were analysed with high performance liquid chromatography and DPH method respectively. Result: PLA_2 activities in the plasma and cell membrane were reduced significantly, and the PS, PE and PC contents in the cell membrane increased significantly in lidocaine group compared with those in endotoxin group. The membrane fluidities of RBC and lung cells of rabbit with lidocaine pretreatment also incresed significantly in comparison with those in endotoxin group. Conclusion:The pretreatment with lidocaine inhibits PLA_2 activity in plasma and cell membrane of rabbit lung, reduces the contents of phospholipids in cell membrane of rabbit lung, increases membrane fluidity of RBC and lung ceils of rabbit and stabilizes cell membranes function.