摘要
测定了3个苏云金芽胞杆菌(Bacillus thuringiensis,Bt)菌株F6、NBT-18、Rt184与绿僵菌(Metarhizium ani-sopliae)菌株HK4复配剂对椰心叶甲致死作用。结果表明,3株Bt菌株对绿僵菌控制椰心叶甲的效果都有不同程度增强。在HK4孢子含量为106个/mL时,F6与HK4、NBT-18与HK4的复配剂处理椰心叶甲,5 d后死亡率分别达71.67%、61.35%,与单独用HK4分生孢子处理(死亡率为30.71%)相比,具有显著差异;而Rt184和HK4复配剂处理的死亡率与HK4单独处理无显著差异。在HK4孢子含量为107个/mL时,3种复配剂处理椰心叶甲5 d后,死亡率分别为82.74%、83.07%、68.61%,均与绿僵菌HK4单独处理(死亡率61.49%)有显著差异;且3个Bt菌株均能使绿僵菌HK4菌株侵染椰心叶甲的致死时间提前。
To investigate the synergistic effect between Bacillus thuringiensis and iVletarhiziwn anisopliae, three B. thuringiensis strains F6, NBT-18 and Rt184, were respectively mixed with M anisopliae strain HK4 for control of Brontispa longissirna (Gestro). The bioassay results showed that all of the Bt strains F6, NBT-18 and Rt184 synergized with IVL anisopliae HK4 to some extent. When the concentration of M.. anisopliae HK4 was 10^6 conidia/mL, the mortality of B. longissima inoculated with the mixture of F6 and HK4 and that of NBT-18 and HK4 was 72% and 61%, respectively, after five days, significantly different from that treated with HK4 only (30%), but there was no significant difference between the mortality caused by Rt184 mixed with or without HK4. When the concentration of M. anisopliae HK4 was 10^7 conidia/mL, the mortality of B. longissima treated with the three mixtures was 82%, 83% and 68%, respectively, after five days, all significantly different from that treated with HK4 only. When M. anisopliae was combined with Bt strains, the lethal time of B. longissima was shortened.
出处
《植物保护》
CAS
CSCD
北大核心
2009年第3期130-132,共3页
Plant Protection
基金
国家科技支撑计划课题(2006BAD08A18)
国家"863"项目(2006AA10A212)
关键词
绿僵菌
苏云金芽胞杆菌
椰心叶甲
协同作用
Metarhizium anisopliae
Bacillus thuringiensis
Brontispa longissirna
synergism