铬中毒性肾损害与铬结合物关系的研究

Study on the relationship between chromium nephrotoxicity and chromium binding substances

本研究采用ＳｅｐｈａｄｅｘＧ５０凝胶层析技术，对铬中毒大鼠肾、尿中铬结合物进行了分离和测定，并分析了各种铬结合物与肾损害的关系。实验结果提示，低分子铬结合物（ＬＭＷ－Ｃｒ）是铬的主要排泄形式，与铬的解毒可能有密切关系；随着肾损害进展小分子铬结合物（ＭＭＷ－Ｃｒ）出现并明显增多，反映了机体形成ＬＭＷ－Ｃｒ能力的紧张，ＭＭＷ－Ｃｒ可能是攻击靶器官的主要活性因素；

Using a subacute chromium induced renal damage animal model,chromium binding substances in urine and kidney were isolated and measured by sephadex G 50 column chromatography and UV AAS The role of different chromium binding substances in the pathogenesis of Cr induced renal injury was discussed The result suggested that a low molecular weight chromium binding substance (LMW Cr) migth play a main role in chromium excretion and detoxification The appearance and increase of a minimal molecular weight chromium binding substance (MMW Cr) seemed to be a signal of the insufficiency of LMW Cr formation,and to be a major active factor on pathogenesis of Cr intoxication Increase of a high molecular weight chromium binding substance(HMW Cr) might be a result of MMW Cr binding with large molecular weight biologic substances