乙乙乳膏对醋酸所致小鼠致痛模型的影响

Effects of YI-yi cream on the Murine Pain Pattern Caused by Acetic Acid

目的:观察乙乙乳膏对醋酸所致小鼠疼痛模型的影响。方法:取小鼠48只,随机分为4组各12只,即乙乙乳膏高、低剂量组、空白组和扶他林对照组。将药物涂于小鼠尾部,面积为1cm2,每天给药1次,连续给药7天,空白组只贴乳膏。于第7天所有小鼠腹腔注射0.6%醋酸溶液0.2mL/10g致痛,记录各小鼠出现第1次扭体反应的时间及15min内扭体次数,比较各组扭体次数。结果:乙乙乳膏高、低剂量组平均扭体次数(16.4±8.5)和(19.2±11.4)次,均显著少于空白组(23.4±12.2)。乙乙乳膏各剂量组与扶他林剂量组(15.8±8.6)次比较,扭体次数无显著差异。乙乙乳膏高、低剂量组扭体反应的抑制率分别为29.9%和19.9%。扶他林组扭体反应的抑制率为32.5%。乙乙乳膏组平均扭体次数均较空白组少(均P<0.01)。结论:乙乙乳膏对醋酸所致小鼠疼痛模型有明显的镇痛作用,且镇痛作用与剂量呈量效关系。

Objective：To investigate effects of Yi-yi Cream on the routine pain pattern caused by acetic acid.Methods ： 48 mice were radomly divided into 4 groups ： Yi-yi Cream high group,Yi-yi Cream low group, empty group and Diclofenae Diethylamine Emulgel group. Mice were all treated for 7 days that put the Yi- yi Cream on the tail ,put the cream on the empty group only. On the seventh day, each mice was injected with 0. 6% HAC 0.2mL/10g to course pain model,and was recorded the number of writhing within 15 minutes and the first writhing. Results： The numbers writhing in the groups of high and low dose of Yi-yi Cream were （16.4 ± 8.5） and （19.2 ± 11.4） , both were significant lower than lhose in-the empty group （23.4±12.2）. Both of the Yi-yi Cream groups of writhing are close to the Diclofenac Diethylamine Emulgel group（15.8 ± 8.6）. Inhibiting rate for writhing in each Yi-yi Cream groups was 29.9% , 19.9%, separately; Inhibiting rate for writhing in Diclofenac Diethylamine Emulgel group was 32.5%. Both of Yi-yi Cream groups were significant lower than empty group （P〈0.01, both）. Conclusion： Yi-yi Cream showed significandy analgesia effect on pain pattern caused by HAC, and which is dose - related.