大豆异黄酮对衰老大鼠抗氧化功能的影响

Effects of Soybean Isoflavones on Antioxidative Action in Aged Rats

目的:研究大豆异黄酮对D-半乳糖致衰老大鼠抗氧化能力的影响。方法:用D-半乳糖注射Wistar雄性大鼠5个月,建立衰老模型。对致衰老模型组、大豆异黄酮组肝脏、心脏和前列腺丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)活性进行测定及比较。结果:低、中、高不同剂量大豆异黄酮灌喂组与模型组大鼠相比,各脏器MDA含量(μmol/L)(心脏:6.95±0.93,5.62±1.12,4.35±1.12比8.02±1.11;肝脏:8.15±0.85,6.47±1.20,5.15±1.12比9.35±1.35;前列腺:7.15±0.92,5.58±1.15,4.23±1.25比8.33±1.24)均有降低,差异有统计学意义(P<0.05),而SOD酶活性(nmol/L)(心脏:47.32±3.08,55.18±4.28,61.20±3.68比32.25±3.70;肝脏:181.21±5.06,190.15±7.06,197.20±5.70比172.13±5.12;前列腺:41.56±3.01,46.07±4.21,50.15±3.35比33.74±3.05)和GSH-Px酶活性(nmol/L)(心脏:9.05±0.96,11.11±2.45,13.13±1.46比7.13±1.51;肝脏:9.02±1.05,11.50±2.23,13.62±1.92比6.98±1.60;前列腺:4.35±0.85,6.13±1.02,7.47±1.55比3.12±1.06)有升高,差异同样具有统计学意义(P<0.05);大豆异黄酮摄入量越高,MDA含量越低,而SOD、GSH-Px酶活性越高。结论:摄入适量大豆异黄酮可有效增强大鼠机体抗氧化能力,从而延缓D-半乳糖诱发的大鼠衰老。

Objective： To study the influence of soybean isoflavones on the antioxidative function of D-gatactose induced aging rat. Methods： Wistar rats were given injections of D-galactose for 5 months to establish aging model. Then they were randomly divided in four groups： model group, low-dose soybean isoflavones group, middle-dose soybean isoflavones group and high-dose soybean isoflavones group whose contents of malondialdehyde （MDA） and the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） of different viscera （liver, heart and prostates） were tested and compared. Resuits： Compared with model group, the contents of MDA （μmol/L） in the hearts （6.95±0.93, 5.62±1.12, 4. 35±1.12 vs 8. 02±1.11）, livers （8.15±0.85, 6.47±1.20, 5.15±1.12 vs 9.35±1.35） and prostates （7.15±0.92, 5.58±1.15, 4.23±1.25 vs 8.33± 1.24） in the low-dose, middle-dose and high-dose soybean isoflavones groups were significantly decreased （P〈0. 05）. However, the activities of SOD （nmol/L） and GSH-Px （nmol/L） in the hearts （47.32±3.08, 55. 184±4.28, 61.20±3.68 vs32.25±3.70; 9.05±0.96, 11.11±2.45, 13.13±1.46 vs 7.13±1.51）, livers （181.21±5.06, 190.15±7.06, 197.20±5.7 vs 172.13±5.12; 9.02±1.05, 11.50±2.23, 13.62±1.92 vs 6.98±1.60） and prostates （41.56±3.01, 46.07±4. 21, 50.15±3.35 vs 33. 74±3.05; 4.35±0.85, 6.13±1.02, 7.47±1.55 vs 3.12±1.06） were significantly raised （P〈0. 05）. The more the soybean intaken, the lower level of MDA and the higher activity of SOD and GSH-Px. Conclusion： Soybean isoflavones can increase antioxidative function in aged rat induced by D-galactose.