凋亡指数在不同宫颈病变组织中的表达及其预测新辅助化疗疗效的作用

Expression of AI in the normal cervical tissue,CIN and cervical squamous carcinoma and their significance

目的:探讨正常宫颈组织、CIN组织及化疗前后宫颈鳞癌组织凋亡指数(apoptosis index,AI)的表达及临床意义。方法:选取11例正常宫颈组织,23例宫颈病变CIN组织,30例宫颈鳞癌组织,应用TUNEL法检测组织AI。结果:化疗临床有效率为80%;正常宫颈组织、CIN组织及化疗前宫颈鳞癌组织中的AI分别为2.55±0.64、14.00±3.16和7.13±1.27(F=127.659,P<0.05),差异有统计学意义;化疗前后宫颈鳞癌组织AI分别为7.13±1.27和17.88±4.39(t=-1.736,P<0.05),差异有统计学意义;化疗前后有效组和无效组宫颈鳞癌组织AI变化值分别为12.21±4.38和4.95±2.69(t=3.849,P<0.05),差异有统计学意义。结论:新辅助化疗能诱导宫颈鳞癌细胞凋亡,AI增高预示疗效较好。

Objective： to study the expression of AI in the normal cervical tissue, CIN and cervical squamous carcinoma and their clinical significance. Methods： From 11 cases of normal cervical tissues, 23 cases of CIN and 30 cases of cervical eancer, AI was assayed by TdT-mediated dUTP-biotin nick end labeling（TUNEL）. Results： Clinical response was 80% （24/30）. The expression of AI were （2.55 ±0.64） in normal cervical tissues , （ 14.00±3.16） in CIN and （7.13 ±1. 27 ） in cervical cancer. The difercnce was significant. The mean AI after NACT was significantly higher in effective cases（ P 〈 0.05）. Conclusion： NACT could increase apoptosis of tumor cell in cervical squamous carcinoma tissue. The change of AI may be one of molecule mec, hanisms leading to anti-tumor function of NACT.