前列腺增生组织中NOS神经的定量分析及意义

Quantitative image analysis of NOS containing nerves in benign prostatic hyperplasia and its clinical significance

为探讨一氧化氮合酶（ＮＯＳ）与前列腺增生（ＢＰＨ）及膀胱出口梗阻（ＢＯＯ）发病的关系，应用ＮＡＤＰＨ组化染色结合形态学定量分析方法检测ＢＰＨ及正常前列腺组织中ＮＯＳ神经，同时分析ＮＯＳ神经与ＢＰＨ临床表现的关系。结果：ＢＰＨ及正常前列腺组织中均存在ＮＯＳ神经，它们主要分布于间质平滑肌细胞周围，ＢＰＨ组织中的ＮＯＳ神经明显减少（Ｐ＜０．０１），前列腺组织中ＮＯＳ神经的长度密度与ＢＰＨ的临床表现相关。

To demonstrate the role of NOS containing nerves in the development of benign prostatic hyperplasia(BPH), NADPH diaphorase histochemistry and quantitative, image analysis were used to quantify the NOS containing nerves in 30 cases of BPH and 20 normal prostatic tissues. The NOS was histochemically localized to nerve fibers and ganglia coursing through the smooth musculature of the stroma. The length density (Lv) of NOS containing nerves in BPH tissues (5.12±3.98) was statistically lower than that in normal prostatic tissues (12.82±6.32) ( P <0.01). A statistically significant correlation was observed between the Lv of NOS containing nerves and the international prostatic symptom score, maximum flow rate, maximum urethral pressure, maximum urethral closure pressure and functional urethral length. No correlation was observed between the Lv of NOS containing nerves and the volume of prostate. Our study indicates that the development of BPH and its secondary bladder outlet obstruction is related to the change of NOS containing nerves in the prostatic tissues.