细胞松弛素D对人类精子顶体反应的影响

The effects of cytocharasin D on human spermatozoal acrosome reaction

为了观察细胞松弛素Ｄ（ＣＤ）对人类精子顶体反应（ＡＲ）的诱导作用，分别使用不同浓度的ＣＤ处理获能后的人精子，以钙离子载体Ａ２３１８７作为阳性对照，采用双重染色法检测精子的ＡＲ发生率。结果当ＣＤ浓度为３０μｍｏｌ／Ｌ及５００μｍｏｌ／Ｌ时，人精子的ＡＲ发生率分别为２８．０±９．０％及２８．６±２．８％，与空白对照组的１７．９±５．５％相比较有明显增加，差异有显著性（Ｐ＜０．０５）。

Cytocharasin D (CD) separated from mold metabolic products mainly affects on the cell microfilament system. In order to observe its induction to human spermatozoal acrosome reaction, different concentrations of CD were used to treat the capacitated human spermatozoal. The double staining technique was adopted as a measurment of the AR rate.Calcium inophore A 23187 was used as a positive control group. It was found that at the concentrations of 30μmol/L CD and 500μmol/L CD the human spermatozoal AR rates were 28.0±9.0%and 28.6±2.8% respectively. There were a significant increase in the AR rate as compared with the plain control group( P <0.05).The results suggest that certain concentrations of CD are a good inducer for human spermatozoal acrosome reaction.