雌激素促进血管瘤血管内皮细胞增殖的体外实验研究

In-Vitro Study of Trophic Effect of Estrogen on Vascular Endothelium of Hemangioma

目的:为研究雌激素对血管瘤血管内皮细胞(VEC_3)增殖的影响。方法:选择雌激素受体阳性的婴儿草莓状血管瘤(例1)和混合型血管瘤(例2)各1例,VEC 按组织块法进行培养。例1VEC 传至第3代时,分为5组:组1、2培养液中不加内皮细胞生长支持物(ECGS),组1为对照组,组2加雌二醇(E_2),组3、4、5培养液中加 ECGS,组3为对照组,组4加 E_2,组5加 E_2和羟基三苯氧胺。于培养第3、6、9天作细胞计数和氚标记胸苷掺入检测。结果:第9天的结果示:组1、2相似,VEC 增殖缓慢。组3 VEC 增殖加快,为组1的2倍(P<0.01)。组4 VEC 增殖较组3更旺盛,为后者的2倍(P<0.01)。而组5 VEC 增殖受到抑制,仅处组1水平。例2分组3、4,结果与例1相似。结论:本实验证实在体外环境:①E_2可促进血管瘤 VEC 增殖。②E_2的促增殖作用有赖于某些生长因子参与。③三苯氧胺可抑制 E_2对血管瘤 VEC 的促增殖作用。

Objective:To assess the effect of estrogen on proliferation of vascular endothelial cells(VEC_3)from hemangioma.Methods:VECs from estogen-receptor positive strawberry he- mangioma and mixed hemangioma were cultured.After three divisions,the VECs from strawber- ry hemangioma were categorized into 5 groups:1)control without endothelial cell growth supple- ment(ECGS),2)estradiol(E_2)without ECGS,3)control with ECGS,4)E_2+ECGS and 5)E_2 +ECGS+OH-tamoxifen.Cell counts and ~3H-TdR incorporations were determined at 3rd,6th and 9th day VECs from the mixed hemangioma were subjected to the treatment of group 3 and 4. Results:On the 9th day,the VECs growth in group 1 and 2 was slow,VECs growth in group 3 doubled that of group 1(P<0.01),and VECs growth in group 4 was further enhanced,being twice that of group 3(P<0.01).In group 5,VECs growth was the same as group 1.The results from mixed hemangioma were similar.Conclusions:The in-vitro study demonstrates(1)E_2 stim- ulates proliferation of hemangioma VECs,(2)the trophic effect of E_2 on VECs depends on certain growth factors and(3)the trophic effect of E_2 can be inhibited by tamoxifen.