聚乳酸/乙醇酸复合bFGF降解支架在心肌血运重建中的应用实验

Experimental studies of degradable PLGA stent with basic fibroblast growth factor in myocardial revascularization

目的探讨高分子缓释降解材料聚乳酸/乙醇酸(poly D,L-lactic/glycolic acid,PLGA)联合碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF),对心肌缺血区域灌注改善的情况。方法成功建立小型猪急性心肌梗死模型后随机分至空白对照组、PLGA支架对照组和PLGA复合bFGF支架组;6周后,SPECT结合软件EmoryCardiac Toolbox分析灌注缺损区域质量百分率的变化,超声学指标LVEDD、LVEDS、FS%评价心脏功能,Image ProPlus软件量化各组新生血管密度。结果6周后PLGA复合bFGF支架组新生血管密度、心肌灌注质量缺损百分率与空白对照组、PLGA支架对照组有显著差异(P<0.01),PLGA复合bFGF支架组FS%较空白对照组、PLGA支架对照组显著改善(P<0.05)。结论bFGF联合PLGA缓释材料能够显著增加新生血管密度,改善缺血部位心肌血流灌注,进而提高心脏功能。

AIM To investigate the effects of degradable poly D, L-lactic/glycolic acid （PLGA） with basic fibroblast growth factor （bFGF） on promoting myocardial revascularization. METHODS Miniswines with acute myocardial infarction were randomly assigned to control group, blank PLGA stent group and PLGA stent with bFGF group. Six weeks postoperatively, quantitative analysis of new vessels was performed by Image Pro Plus software. The mass defect percent of myocardial perfusion was calculated by Emory Cardiac Toolbox software. LVEDd, LVEDs and FS% reflective of cardiac function were meas- ured by echocardiology. RESULTS Six weeks postoperatively, both new vessels density and mass defect percent in PLGA stent with bFGF group showed significant difference compared with those in control group and blank PLGA stent group （P 〈0. O1 ）. FS% in PLGA stent with bFGF group was significantly enhanced compared with that in the other two groups （P 〈 0. 05）. CONCLUSION bFGF combined with degradable PLGA stent increases new vessel＇s density, improves myocardial perfusion and enhances cardiac function.