大蒜油对正己烷在小鼠体内代谢成2，5-己二酮的影响

Effects of garlic oil on n-hexane metabolized to 2,5-hexanedione in mice serum

目的 探讨大蒜油对正己烷（n—hexane）在小鼠体内代谢成2，5-己二酮（2，5-hexanedione，2，5-HD）的影响。方法 取健康成年昆明种小鼠，随机分为正己烷染毒组和大蒜油干预组，分别灌胃给予正己烷和大蒜油，染毒结束后取血，分离血清经乙酸乙酯萃取后气相色谱法测定血清中2，5-HD含量。结果 （1）一次性给予4000mg／kg正己烷后，小鼠血清中2，5-HD含量随时间延长而增加，10h后达峰值，20h后几乎不能检出；（2）对照组小鼠血清中未检出2，5-HD；随着正己烷染毒量的增加，小鼠血清中2，5-HD含量明显增加，2000、4000和6000mg／kg组染毒8h后小鼠血清2，5-HD含量分别为8．04、16．68和22．38μg／ml，呈明显的剂量一效应关系；（3）不同周龄小鼠给予正己烷8h后血清中2，5-HD含量有明显差异，5周龄组（22．83μg／mJ）分别大于4周（19．59μg／m1）和6周龄组（16．42μg/ml），差异有统计学意义（P〈0．05）；（4）不同性别小鼠给予同剂量正己烷后，雌性小鼠血清中2，5-HD含量（13．22μg／ml）高于雄鼠（10．34μg／m1），差异有统计学意义P〈0．05；（5）正己烷染毒前后2h分别给予80mg／kg大蒜油，血清中2，5-HD含量与单纯染毒组相比均明显降低，差异有统计学意义（P〈0．05），但染毒前给药组较染毒后给药组更低，但差异无统计学意义（P〉0．05）；染毒前2h给予不同剂量大蒜油，10、20、40、80mg／kg大蒜油＋3000mg／kg正已烷组小鼠血清2，5-HD含量比染毒组分别降低16．2％、20．8％、22．8％和32．1％，差异有统计学意义（P〈0．05，P〈0．01），呈明显的剂量-效应关系。结论 在雌性小鼠血清中正己烷的代谢产物2，5-HD高于雄性；5周龄小鼠血清2，5-HD含量较4周和6周龄小鼠高；正己烷染毒前后给予大蒜油可明显减少2，5-HD的生成。

Objective To investigate the effects of garlic oil （GO） on n-hexane metabolized to 2,5- hexanedione（2,5-HD） in mice. Methods Adult healthy Kunming-mice were treated with n-hexane and GO. The serum was obtained and extracted with ethyl acetate, and the levels of the serum 2,5-HD were determined by gas chromatography. Results （1） The concentration of 2,5-HD in serum increased firstly after a single exposure to n-hexane （4 000 mg/kg）. The peak value occurred at 10 hours after n-hexane treatment, but could hardly be detected at 20 h. （2） There was no 2,5-HD in serum of control mice. The content of 2,5-HD in serum increased along with the exposure dose of n-hexane. The serum 2,5-HD contents of the 2000, 4000 and 6000 mg/kg groups mice were 8.04, 16.68 and 22.38 μg/ml at 8 h in pretreated mice, respectively, and showed significant dose-effect relationship.（3） When the different age mice were exposed to the same dose of n-hexane, the contents of 2,5-HD in serum were significantly different after 8 hours （P〈 0.05）. The serum 2,5-HD level of the 5 weeks old mice （22.83μg/ml） was much higher than the 4 （19.59 μg/ml） and 6 （16.42 μg/ml） weeks old mice. （4） When the different gender mice were exposed to the same dose of n-hexane, the concentration of 2,5-HD in serum of female mice （13.22 μg/ml） was higher than that of the female mice （10.34μg/m], P〈0.05）. （5）GO significantly inhibited the increase of the serum 2,5-HD levels of both the pretreatment and post-treatment groups treated with 80 mg/kg n-hexane respectively, but the pretreatment with GO exhibited the more suppressive effects than the post-treatment （P〉0.05）. Compared with the n-hexane group, the concentrations of serum 2,5-HD in GO-pretreated groups mice decreased by 16.2%, 20.8%, 22.8%（P〈0.05） and 32.1% （P〈0.01）, respectively, and showed significant dose-effect relationship.Conclusion The serum content of 2,5-HD, the metabolite of n-hexane, is different in different genders and age mice after exposed to the same dose of n-hexane. GO can effectively inhibit the production of n-hexane metabolized to 2,5-HD in mice serum.