流式细胞术检测职业铅接触工人的DNA损伤

Detection of DNA damage of workers occupationally exposed to lead with flow cytometer assay

目的 用γH2AX识别抗体流式细胞术（FCM）研究铅暴露对人体外周血淋巴细胞DNA的损伤作用。方法 淋巴细胞采自41例铅作业工人和50例对照人群，应用FCM检测淋巴细胞DNA损伤情况。结果 不同铅接触水平工人外周血淋巴细胞DNA损伤率和几何平均荧光强度明显高于对照组，差异有统计学意义（P〈0．05）；不同工龄组工人DNA损伤率和几何平均荧光强度差异无统计学意义（P〉0．05）；DNA损伤率与血铅、尿铅、δ-氨基乙酰丙酸（ALA）相关性分析无统计学意义（P〉0．05），几何平均荧光强度与血铅、尿铅、δ-ALA相关性分析无统计学意义（P〉0．05）；高浓度组血铅、δ-ALA与DNA损伤率呈正相关（r值分别为0．681、0．684），高浓度组血铅与几何平均荧光强度呈正相关（r值为0．795），差异有统计学意义（P〈0．05）。结论 职业性铅接触可引起人体外周血淋巴细胞DNA损伤，FCM是一种检测外周血淋巴细胞DNA损伤的有效方法。

Objective To study DNA damage of workers occupationally exposed to lead with flow cytometer assay. Methods The lymphocytes were obtained from 41 workers occupationally exposed to lead （comparable group） and another 50 from control group. Flow cytometer （FCM） assay was used to detect DNA damage. Results DNA damage rate and geometric mean fluorescence intensity in the comparable group were significantly higher than those in the control group （P〈0.05）. There were no significant differences in the DNA damage and geometric mean fluorescence intensity between different age groups （P〉0.05）. The differences in correlation analysis between blood lead, urine lead,δ-ALA and DNA damage rate were not significant （P〉0.05）. The correlation analysis showed no statistical significance between concentration of blood lead, urine lead ,δ-ALA and geometric mean fluorescence intensity （P〉0.05）. There was positive correlations not only between the high concentration of blood lead, δ-ALA and damage rate of DNA, but also between the high concentration of blood lead and geometric mean fluorescence intensity. The coefficient r showed statistical significance（P〈0.05 ）. Conclusion Occupational lead exposure can cause DNA damage. γH2AX flow cytometer assay is a sensitive, objective and effective method for detection of DNA damage of peripheral blood lymphocytes.