胆汁引流对胆源性胰腺炎大鼠血浆脑肠肽水平和胰腺病理变化的影响

Effect of bile-shunt on the level of plasma braingut peptide and the changes of pancreatic morphology in rats with biliary pancreatitis

分别观察了胆胰管结扎大鼠(A组)、胆胰管结扎后30分钟加胆汁外引流(B组)、胆胰管结扎后30分钟加胆汁内引流(C组)及对照大鼠(D组),在不同时期检测大鼠血浆胆囊收缩素八肽(CCK-OP)、生长抑素(SS)及血清淀粉酶水平和胰腺重量及组织学记分.结果:术后3小时,A、B、C组血浆CCK—OP、SS及血淀汾酶含量均明显高于D组(P<0.05);B组高于A、C组(P<0.05);C组低于A组(P<0.05);A、B、C组在术后3小时,血浆CCK-OP及SS含量均达高峰,A、C组血浆CCK-OP、SS在术后6小时及24小时均下降至正常,B组仍高于D组;A、B、C组胰腺均呈急性炎症改变;A、B组胰腺组织学记分及重量均明显高于C组(P<0.05);B组又高于A组(P<0.05).

The concentration of plasma cholecystokinin-octapeptide (CCK-OP) , somatostatin (SS), serum amylase and the pancreatic histologic score were measured in the rats of 4 groups:group A were subjected to bile pancreatic duct obstructtion;group B bile pancreatic duct obstruction with bile internal-shunt; group C bile pancreatic duct obstruction with bile external-shunt and group D sham operation (dissection,no obstruction). The results showed that 3 h after the experiment,the contents of plasma amylase,CCK-OP,and SS were incresed obviously in groups A,B and C as compared with group D(P<0. 05). The contents of CCK-OP were peak at 3rd h after operation and returned to normal level in groups A and C,and abnormal in group B at postoperative 6th h and 24th h. The histologic scores and the weight of pancreases were increased significantly in group A,B and C as compared with group D(P<0. 05) ,in group B as compared with group A(P< 0. 05). It was suggested that bile external-shunt was associated with increases of plasma CCK-OP,SS and pancreatic histologic scores in rats with biliary pancreatitis.