甘氨脱氧胆酸诱导肝癌SMMC-7721细胞凋亡中对P53表达的影响

The effect of glycodeoxycholic acid on P53 expression in the apoptosis of SMMC-7721 cells

目的探讨甘氨脱氧胆酸(GDCA)在诱导SMMC-7721细胞凋亡中对P53表达的影响。方法用膜联蛋白V(annexinV)检测细胞凋亡,RT-PCR分析p53 mRNA变化,免疫组化技术分析P53蛋白表达。结果GDCA可明显诱导SMMC-7721细胞凋亡,具有明显的时间和剂量依赖。200μmol/L GDCA处理组在48 h和72 h的细胞凋亡率分别为(7.67±2.35)%、(12.93±1.48)%;400μmol/L GDCA处理组则分别为(13.14±2.56)%、(14.22±2.11)%。GDCA可明显提高p53 mRNA水平,且较强地抑制突变的P53蛋白的表达。结论GDCA具有诱导SMMC-7721细胞凋亡作用。

Objective To investigate the effect of glycodeoxycholic acid （GDCA） on apoptosis of and P53 expression in SMMC-7721 cells. Methods Apoptotic rate was examined by Annexin V. P53 mRNA was analyzed by RT-PCR, and P53 protein was measured by immunohistochemistry technique. Results Compared with the control, SMMC-7721 cells treated with GDCA had significantly increased apoptotic rate in a dose- and time-dependent manner. After treated with 200 μmol/L GDCA, the apoptotic rates of the cells at 48 h and 72 h were （7.67 ± 2.35）% and （12.93 ± 1.48）% respectively. When the GDCA concentration was raised to 400 μmol/L,the apoptotic rates of the cells at 48 h and 72 h also increased to （ 13. ld ±2.56）% and （ 14.22 ±2.11 ）% respectively. The p53 mRNA in SMMC-7721 ceils was up-regulated,whereas the mutant P53 protein was obviously inhibited after the cells were treated with GDCA. Conclusions GDCA can induce the apoptosis of SMMC-7721 cells.