外周血淋巴细胞泛素mRNA的表达与慢性乙型肝炎病情的关系

Association of the ubiquitin mRNA expression in peripheral blood lymphocytes and chronic hepatitis B

目的探讨外周血淋巴细胞泛素mRNA的表达与慢性乙型肝炎病情的关系。方法87例慢性乙型肝炎患者,按病情分为轻(26例)、中(33例)、重(28例)3组,同时设立健康对照组(26例)。用实时荧光PCR检测外周血淋巴细胞泛素mRNA及内参照β-actin的表达;用电化学发光法定量检测HBeAg和抗HBe;用荧光定量PCR检测血清HBV DNA。结果慢性重度和慢性中度肝炎患者外周血淋巴细胞泛素mRNA水平明显低于健康对照,均值分别为健康对照的68.78%和77.67%(P<0.05),慢性轻度肝炎患者与健康对照比较差异无显著性;泛素mRNA水平与患者HBeAg和HBV DNA水平呈负相关(P<0.05),与抗HBe水平无相关。结论慢性乙型肝炎患者存在泛素表达不足,泛素表达水平与HBV复制和疾病的严重程度相关,病情越重泛素表达水平越低。

Objective To study the relationship between the ubiquitin mRNA expression in peripheral blood lymphocyte and chronic hepatitis B. Methods Based on the disease severity, 87 chronic hepatitis B patients were divided into： mild （26）, moderate （33）, and severe （28） groups. Healthy persons （26） served as control. The ubiquitin mRNA of peripheral blood lymphocytes was detected by real-time PCR using β-actin as an intra-referenee. Electrochemiluminescence was used to quantitate HBeAg and anti-HBe. Fluorescent quantitation PCR was applied to detect serum HBV-DNA. Results The expression levels of ubiquitin mRNA in moderate and severe groups were obviously lower than that in the control group （68.78% and 77.67% respectively,P 〈0.05）, while those in mild and control group were comparable. The ubiquitin mRNA expression was negatively correlated with the levels of HBeAg and HBV DNA （P 〈0.05 ）, yet not correlated with anti-HBe. Conclusions The expression of ubiquitin mRNA is reduced in chronic hepatitis B patients, which is correlated with disease severity and HBV replication.