吸烟和年龄对电子垃圾拆解工人尿液中8－羟基脱氧鸟苷的影响

Smoking status or age impact on urinary 8-hydroxy-2＇-deoxyguanosine in electrical and electronic equipment waste dismantling workers

目的研究吸烟和年龄对电子垃圾拆解工人尿液中8-羟基脱氧鸟苷（8-hydroxy-2’．deoxyguanosine，8-OHdG）的影响。方法采用固相萃取-高效液相色谱电化学检测器（SPE-HPLC—ECD）方法，检测64名电子垃圾拆解男性工人上班前和下班后尿液中8-OHdG浓度。按照吸烟情况和工人的年龄分别对数据进行统计分析。结果相对于吸烟者（42名），不吸烟者（22名）尿中8-OHdG浓度明显的增高，上班前，不吸烟组的工人尿液中的8-OHdG浓度为（8．25±4．23）μmol／mol肌酐，吸烟组工人尿液中8-OHdG浓度为（5．44±1．18）μmoL／mol肌酐，两者差异无统计学意义（t=-0．81，P=0．42）。而在下班后，不吸烟组的工人尿液中的8-OHdG浓度为（43．12±16．19）μmol／mol肌酐，大约是吸烟组工人尿液中8-OHdG浓度[（14．82±2．51）μmol／mol肌酐]的3倍。经过1d暴露下班后，吸烟和不吸烟工人尿液中的8-OHdG浓度差异具有统计学意义（t=-2．33，P〈0．05）。按照工人的年龄分组，上班前，19岁～（6名）、20岁～（22名）、30岁～（23名）、40～49岁（11名）组工人尿中8-OHdG浓度分别为（1．86±0．66）、（3．57±0．54）、（8．12±4．10）、（11．39±3．70）μmol／mol肌酐，组间差异没有统计学意义（F=0．98，P=0．41）；但经过1d的暴露，下班后，19岁-、20岁～、30岁～、40—49岁组工人尿中8-OHdG浓度分别为（4．19±2．85）、（19．89±5．26）、（28．89±14．61）、（34．94±12．50）μmol／mol肌酐，组间差异具有统计学意义（F=4．81，P=0．03）。结论吸烟对拆解工人尿中8-OHdG浓度有一定的抑制作用，尿中8-OHdG浓度随年龄增高而增加。

Objective To investigate the smoking or age impact on occupational workers in electrical and electronic equipment waste （e-waste） dismantling procedure, using 8-Hydroxy-2＇- deoxyguanosine （8-OHdG） in the urine as a biomarker for oxidative damage to DNA. Methods The pre-workshift and post-workshift urinary samples of 64 male workers in e-waste dismantling procedure were detected by solid-phase extraction-high performance liquid chromatography-electrochemical detector （SPE-HPLC-ECD）. The data were statistically analyzed by two factors, age or smoking status. Results The 8-OHdG levels in non-smokers＇ urines （ n = 42 ） were higher than those in smokers＇ urines （ n = 22 ）. The levels in pre-workshift urines were detected at （ 8. 25 ± 4. 23 ） μmol/mol creatinine in non-smokers, while the values were （ 5.44 ± 1.18 ）μmol/mol in smokers. And, the levels in post-workshift were detected at （43.12 ± 16. 19） μmol/mol creatinine in non-smokers,while the values were（ 14. 82 ± 2. 51 ） μmol/mol in smokers. The 8-OHdG levels in pre-workshift urines were not different between non-smokers and smokers （ t = - 0. 81, P = 0. 42 ） , however after 1 day exposure, urinary 8-OHdG levels were significantly increased in non-smokers than those in smokers （ t = - 2. 33, P 〈 0. 05 ）. On the other hand, the subjects were divided into five groups according to their age. The 8-OHdG levels in pre-workshift urines were （ 1.86 ± 0. 66）, （ 3.57 ± 0. 54 ）, （ 8. 12±4. 10 ）, （ 11.39±3.70 ） μmoL/mol creatinine in 〈 20 years group （ n = 6 ）, 20 - years group （ n = 22 ）, 30 - years group （ n = 23 ） ,40 - 49 years group （ n = 11 ） respectively. No effect of age was found on the pre-workshift urinary 8-OHdG levels （F =0. 98 ,t =0. 41 ）. However,it was found that the post-workshift urinary 8-OHdG levels increased along with the e-waste workers＇ age （ F = 4. 81, P = 0.03） ,and they were （4. 19 ± 2.85）, （19.89 ± 5.26）, （28.89 ± 14.61）, （34.94 ± 12.50） μmol/mol creatinine in 〈 20 years group, 20- years group, 30 - years group, 40 -49 years group respectively. Conehltsion The urinary 8-OHdG levels in the e-waste dismantling workers might be inhibited by smoking status. The oost-workshift urinary 8-OHdG levels increased alone with the e-waste warkers＇ age.