胶体金与间接血凝方法在鼠疫疫区回顾性调查中的效果评价

The evaluation to colloidal gold and IHA method's application in the investigation of retrospective to plague-affected areas

目的了解胶体金快速检测试剂盒在鼠疫疫区回顾性调查中的应用效果。方法按鼠疫胶体金快速检测试剂盒的使用说明进行,并用间接血凝(IHA)法进行比较。结果检测了采集于康保县鼠疫疫源地内血清共195份,间接血凝检出阳性血清4份,胶体金检出阳性血清3份。对照组血清14份,结果均为阴性。结论胶体金快速检测试剂盒在鼠疫疫区回顾性调查中与间接血凝阳性检出率无明显差异,但胶体金试剂盒的敏感性明显低于间接血凝试验,在用于疫区回顾性调查时,应附加间接血凝试验佐证。

Objective To understand the application effect of the colloid gold fast examination reagent box in the re- view investigation of plague epidemic areas. Methods According to the instructions of the colloid gold fast examination reagent box, and make a compare with the indirect hemagglutination assay. Results Examined 195 blood serum of plague epidemic ar- eas in Kangbao County, as a result ,4 masculine blood serum with the IHA, 3 masculine blood serum with the colloid gold. Ex- amined 14 blood serum of the control group , the result of all is negative. Conclusions The colloid gold fast examination reagent box can be used in the review investigation of plague epidemic areas, and there is no difference with the IHA in statistics , but the colloid gold^ensibility is below the IHA,so we should make the IHA support the colloid gold at the same time.