罗格列酮对急性冠脉综合征患者外周血单核细胞ABCA1蛋白表达的干预

The interventional effect of rosiglitazone on the expression of protein ABCA1 in peripheral blood mononuclear cells in patients with acute coronary syndrome

目的:研究罗格列酮对急性冠脉综合征(Acutecoronary syndrome,ACS)患者外周血单核细胞ABCA1蛋白表达的影响。方法:选取ACS患者21例,密度梯度法分离外周血单核细胞。实验分为:(1)不同终浓度(0μmol/L、1.0μmol/L、10μmol/L、100μmol/L)罗格列酮分别与单核细胞孵育24h。(2)选定10μmol/L终浓度罗格列酮分别与单核细胞孵育不同时间(0h、6h、12h、24h)。(3)PBS对照组。每组单核细胞各培养3孔为测定孔,均同时设空白1孔。孵育结束后用流式细胞技术检测单核细胞ABCA1蛋白的表达水平。结果:与对照组比,单核细胞在罗格列酮作用后,其ABCA1蛋白表达呈浓度和时间依赖性增加。结论:罗格列酮能提高ACS患者外周血单核细胞ABCA1蛋白的表达,可能为罗格列酮抗动脉粥样硬化的重要机制之一。

Objective： To investigate the interventional effect of rosiglitazone on the expression of protein ABCA 1 （ ATP-binding cassette transporter-1 ） in peripheral blood monocytes（ PBMC ） in patients with acute coronary syndrome（ACS）. Methods： Twenty-one patients with ACS were enrolled in the study. PBMCs were isolated by density gradient centrifugation. The experiments included： （1） Rosiglitazone of different final concentrations（0μmol/L, 1.0μmol/L, 10μmol/L, 100μmol/L）were incubated with PBMCs for 24h. （2） Rosiglitazone of the chosen final concentration of 10μmol/L was incubated with PBMCs for 0 h,6 h, 12 h and 24 h,separately.（3） Control groups were also set （cells treated with PBS）.Three wells served as measured wells. And one well acted as blank well in each group.Then the level of protein ABCA 1 was determined by flow cytometry after incubation. Results： Compared with control groups, after PBMCs were intervened by rosiglitazone, the expression of protein ABCA1 of PBMCs increased in dose-and timedepe-ndent manners.Conclusion： Rosiglitazone can increase the expression of protein ABCA1 of PBMCs,which may be one of the possible mechanisms for rosiglitazone to play an anti- atherosclerotic role.