CAPE对神经胶质瘤生长抑制作用研究

Effects of caffeic acid phenethyl ester in wnt/β-catenin signaling pathway on human neuroglioma

目的:探讨咖啡酸苯乙酯(Caffeic acid phenethyl ester,CAPE)在人神经胶质瘤中的治疗作用。方法:建立裸鼠胶质瘤模型,观察CAPE在体内对肿瘤的影响。运用实时荧光定量PCR、Westernblotting检测CAPE对胶质瘤细胞株U251异常Wnt/β-catenin通路及靶基因c-myc的影响。结果:通过对荷瘤鼠模型的观察,发现口服CAPE可以抑制神经胶质瘤的生长。CAPE可以抑制β-catenin基因mRNA及蛋白的表达,下游靶基因c-myc的mRNA及蛋白表达也相应降低。结论:CAPE可以抑制神经胶质瘤异常Wnt/β-catenin通路中β-catenin基因及c-myc基因的表达,对神经胶质瘤的生长有抑制作用。

Objective： The therapic effect of caffeic acid phenethyl ester （ CAPE ） in neuroglioma was to be investigated in this article. Methods： Athymic mouse model was constructed for therapic observation when tumor was treated by CAPE in vivo. Then Real-time PCR was employed to detected the expressing level of c-myc and β-catenin genes in Wnt/β-eatenin signaling pathway when U251 cells were treated by CAPE. Results： CAPE could inhibit the growth of neuroglioma in athymic mouse through drug oral application. The mRNA and protein expression of β-catenin were inhibited. Furthermore, c-myc gene, which was the downstream of β-catenin gene, was also down-regulated after the drug treatment. Conclusion： The expressing level of β-catenin and e-mye genes were down-regulated after CAPE treatment, by which the growth of neuroglioma could be inhibited.