蛋白质溶液结构及动力学的核磁共振研究

NMR Studies of Protein Solution Structures and Dynamics

高场液相核磁共振技术作为解析高分辨率蛋白质结构的两大主要手段之一,在近二十几年的时间里得到了迅猛的发展.一方面,随着谱仪硬件技术、核磁脉冲技术和蛋白质标记技术的不断发展,液相核磁共振技术所能够研究的蛋白质不断突破分子量的限制,可以达到几万,甚至几十万.另一方面,液相核磁共振技术成功地应用于蛋白质分子动力学的研究中,是目前唯一能够对蛋白质多个位点同时进行动力学研究的实验方法,并且仍在不断地创新、发展和完善中.本文从蛋白质溶液结构的解析和动力学的研究两个主要方面对液相核磁共振研究的基本方法进行简要的介绍,并结合实例介绍一些最新的研究进展.

As one of the major techniques for determining high-resolution protein structures, high-field solution NMR has achieved remarkable developments during the past twenty years. With the advances of advanced spectrometer hardware, NMR pulse techniques and protein labeling strategies, the size of the protein that can be studied by solution NMR now exceeds 25 k, and can reach tens and even hundreds of kilo Dalton. In addition, solution NMR has also been widely used in the studies of protein dynamics, and is the only technology that can be used to probe protein motions at multiple sites simultaneously. In this review, we briefly summarize the methods for studying protein structures and dynamics using solution NMR, accompanied by examples to demonstrate the latest developments in the field.