粉防己碱抗豚鼠再灌注早期心室颤动及其机制

Effect and Mechanism of Tetrandrine on Ventricular Fibrillation at Early Phase of Reperfusion in Guinea Pig

目的：探讨粉防己碱抗豚鼠再灌注早期心室颤动作用及其机制。方法：利用心肌缺血再灌注损伤模型，研究粉防己碱对再灌注早期心脏去甲肾上腺素（ＮＡ）释放及心室颤动发生的影响。结果：①粉防己碱使心脏流出液中ＮＡ含量从每克心脏组织３．７２±０．９６ｐｍｏｌ／ｍｉｎ减低为０．７８±０．３６ｐｍｏｌ／ｍｉｎ（ｎ＝７，Ｐ＜０．００１）。②粉防己碱可明显降低再灌注早期心室颤动的发生（Ｐ＜０．０５）。结论：粉防己碱可明显减少再灌注早期心交感神经递质ＮＡ的释放，同时可明显减少心室颤动发生。该减少再灌注早期心交感神经递质ＮＡ释放的作用应是抗再灌注性心律失常—心室颤动作用机制之一。

Objective:To study the effect and mechanism of tetrandrine(Tet) on ventricular fibrillation at the early phase of reperfusion in guinea pig. Methods:The model of heart reperfusion injury was used to investigate the effect of Tet on cardiac norepinephrine(NA) release and ventricular fibrillation at the early phase of reperfusion. Results:①Tet decreased the NA overflow from 3 72±0.96 to 0.78±0.36 pmol/(min·g)( n =7, p <0.001).②Tet reduced the incidence of ventricular fibrillation significantly( p <0.05). Conclusion:These results suggest that the decrease of NA relaese by Tet contributes to its anti ventricular fibrillation effect at the early phase of reperfusion.