RP-HPLC法测定杞菊地黄片中丹皮酚的含量

Determination of Paeonol in Qijudihuang tablet by RP-HPLC

【目的】建立RP-HPLC法测定杞菊地黄片中丹皮酚含量的方法。【方法】以KromasilC18液相柱（250mm×4．6min，5μm）为固定相，甲醇-0．05磷酸溶液（70：30）为流动相，检测波长为274nm，流速为1．0ml／min。【结果】丹皮酚在50～800ng范围内线性关系良好（r=0．9999），平均加样回收率为99．55（n=2）；测得杞菊地黄片中丹皮酚的含量为1．804mg/片。【结论】RP-HPLC法专属性强，重复性好，可用于杞菊地黄片的质量控制。

[ Objective] To establish a method for the determination of Paeonol in Qijudihuang tablet by RP- HPLC. [Methods] The chromatographic conditions were Kronmsil C18 （250 mm× 4.6 mm, 5 μm） as analytic column, methano-0.05% orthophosphoric Acid （70：30） as mobile phase, the flow rate being 1.0 ml/min, the detection wavelength at 274 nm. [Results] The linearity of Paeonol in the range of 50 - 800 ng was good, r = 0. 9999. The average recovery was 99.5% , and the content of Paeonol in Qijudihuang tablets was 1.804mg every tablet. [ Conclusions] The RP- HPLC method is accurate, reliable, specific, and can be used for the quality control of Qijudihuang tablet.