摘要
Objective： To investigate the expression of hergl gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K＋ channel expressions and tumor cell proliferation and apoptosis. Methods： RT-PCR and PCR assays were used to detect the expression of hergl gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K＋ channels was used to evaluate their effects on tumor cell proliferation and apoptosis. Results：The statistically significant expression of hergl gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K＋ channel blocker, E-4031, increased the cell population in G0/G1（P 〈 0.05） and the number of apoptotic tumor cells（P 〈 0.05）. Conclusion： HERG K＋ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.
Objective： To investigate the expression of hergl gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K＋ channel expressions and tumor cell proliferation and apoptosis. Methods： RT-PCR and PCR assays were used to detect the expression of hergl gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K＋ channels was used to evaluate their effects on tumor cell proliferation and apoptosis. Results：The statistically significant expression of hergl gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K＋ channel blocker, E-4031, increased the cell population in G0/G1（P 〈 0.05） and the number of apoptotic tumor cells（P 〈 0.05）. Conclusion： HERG K＋ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.
基金
supported by a grant from the Natural Science Foundation of China(30772128)
