PAEs对淋巴细胞分泌IL-4蛋白的影响

Effect of PAEs on IL-4 production in primary lymphocyte

目的:以DBP和DEHP为例,研究PAEs对淋巴细胞分泌IL-4蛋白的影响,为进一步探讨PAEs的免疫毒性提供基础。方法:分别用ConA及PMA+Ion去激活脾淋巴细胞,以不同浓度DBP和DEHP染毒细胞72 h、96 h,实验终点用ELISA方法检测细胞上清液中细胞因子IL-4蛋白表达。结果:不同激活条件下,10μM、50μM的DBP或DEHP作用72h均明显降低细胞上清液中IL-4蛋白表达;而10μM的DBP或DEHP作用96h却显著促进了IL-4蛋白表达。结论:不同激活条件下,PAEs对淋巴细胞分泌IL-4蛋白影响不同,可能与其免疫毒性有关。

Objective： To study the effect of PAEs （DBP and DEHP） on IL-4 production in primary lymphocyte Methods： ConA and PMA （Phorbol Ester） ＋ Ion （Ionomycin） were used to activate the lymphocytes respectively. The primary lymphocyte were exposed to DBP or DEHP for 72h and 96h. At the end of the experiment, IL-4 protein was detected in supernatant by ELISA method. Results： 10μM, 50μM DBP or DEHP reduced significantly IL-4 content in 72h under different activated condition. 10μM DBP or DEHP increased obviously IL-4 in 96h. Conclusion Under different activating conditions, PAEs express different effect on IL-4 protein, which may be related to its immune toxicity.