梅花鹿3个种群遗传多样性的微卫星标记分析

Genetic Diversity in Three Sika Deer Populations as Revealed by Microsatellite Markers

利用16个微卫星标记对黑龙江省部分地区（兴凯湖农场、大庆市银浪牧场、五大连池大庆农场鹿苑）的3个梅花鹿（Cervus nippon）群体进行了遗传多样性检测。统计了3个鹿群的等位基因组成、平均有效等位基因数（Ne）、平均遗传杂合度（h）和多态信息含量（PIC）。结果表明,除5个位点外,其余11个微卫星位点均表现出不同的多态信息含量,其中高度多态位点5个,中度多态位点4个。这说明本研究所选用的微卫星位点可较准确地评估3个梅花鹿群体的遗传多样性,并为今后相关研究筛选出了有价值的引物。3个梅花鹿群体的平均h在0.454-0.636之间变动,其中兴凯湖梅花鹿群体最高,为0.636,具有较大的遗传潜力。

The genetic polymorphism of 16 microsatellite loci in three Sika Deer （Cervus nippon ）populations in Heilongjiang Province （Xingkaihu farm in Mishan region, Yinlang livestock farm in Daqing region, and Daqing farm in Wudalianchi region） was determined. Number of alleles, effective number of alleles （ Ne ）, mean heterozygosity （ h ） and polymorphism information content （PIC） were calculated for each population. The results showed that 11 of 16 microsatellite loci were polyrnorphic except for TS01, C14, C217, T107 and C273.Among the 11 loci with polymorphism, five were highly polymorphic and four were medium polymorphic, showing that these microsatellite loci are effective markers for the genetic analysis of C. nippon populations. The average heterozygosity of the three groups changed from 0.453 to 0.636 and Xingkaihu Group had the highest h （0.636）,which could have more potential for selection.