体外MTT药敏试验在指导原发性肝癌化疗中的作用

Application of MTT to Liver Carcinoma in vitro Drug-sensitivity Testing

目的探讨3-(4,5)-双甲基-2-噻唑-(2,5)-二苯基溴化四氮唑蓝(MTT)药敏试验法在指导临床原发肝癌化疗中的作用。方法45例原发肝癌标本制成单细胞悬液,分为MTT敏感组及经验对照组分别进行细胞培养,MTT敏感组按MTT试验药敏检测结果组成化疗方案给药,经验对照组凭经验及文献用药。计算各药物对肿瘤细胞相对抑制率IR及敏感率,分析结果。结果(1)肝癌细胞对不同化疗药物的敏感性不同。(2)MTT敏感组肝癌细胞对化疗药物的敏感率明显高于经验对照组肝癌细胞对化疗药物的的敏感率。结论MTT法对原发性肝癌的评估能为原发性肝癌的个体化疗提供参考。

Objective The predictive value of MTT assay in vitro for chemotherapy to liver cancer cells was studied. Methods 11 chemotherapeutical agents were used to exam the effective rates of inhibition of 45 liver cancer cells by MTT assay in vitro. According to the result of the MTT assay, the cells of 45 liver cancer patients received sensitive chemotherapeutical agents. And the cells in the control group were given chemotherapeutical agents according to clinical experience. Results （ 1 ） The liver cancer cells had a different sensitive chemotherapeutical agent table. （2）There was statistically significant difference in sensitive group as compared to that in control group. Conclusion Evaluating the liver cancer sensitivity of chemotherapeutical agents by MTT assay can be benefit its individualized chemotherapy.