摘要
目的：建立猪殃殃药材中绿原酸的薄层色谱鉴别与含量测定方法。方法：采用TIE法对猪殃殃进行鉴别；采用HPLC法测定绿原酸含量，色谱条件为：色谱柱：Phenomenex Luna C18（4．6mm×150mm，5μm）；流动相：乙腈-0．4％磷酸溶液（10：90）；检测波长：327nm；流速：1．0ml/min；柱温：35℃。结果：各批猪殃殃药材TLC色谱中均能检出绿原酸；绿原酸眦色谱峰与其它色谱峰分离良好，进样量在0．0892-0．4460μg范围内，呈良好线性关系（r=0．9997），平均回收率为96．99％，RSD=1．17％（n=6）。结论：本法简便快捷，结果可靠．为控制猪殃殃药材的质量提供参考。
Objective： To establish methods for determination and the TLC identification of chlorogenic acid in Galium aparine. Methods：TLC and HPLC were adopted to identify and determine Galium aparine. The chromatographic conditions were： Phenomenex lama C18 （2）, （4.6mm × 150mm,5μm）column as analytic column; Acetonitrile- 0.4%H3PO4 water solution （10：90） as mobile phase; detection wavelength at 327nm; column temperature at 35℃ ;flow rate at 1. 0ml· min^- 1. Results： chlorogenic acid was identified by TLC and determined by HPLC. The liner range of chlorogenic acid was 0. 0892 - 0. 4460μg, r = 0. 9997. The average, recovery was 96.99%, RSD = 1.17%. Conclusion： These methods are simple, accurate, reproducible and can be used to enhance the quality control of this drug.
出处
《中国民族医药杂志》
2009年第5期40-42,共3页
Journal of Medicine and Pharmacy of Chinese Minorities
关键词
猪殃殃
薄层色谱鉴别
绿原酸
高效液相色谱法
Galium apafine
TLC identification
Chlorogenic acid
HPLC
determination
