不同给药途径的腺苷预处理对大鼠缺血再灌注心肌的保护

Protective effect of adenosine preconditioning on myocardial ischemia reperfusion injury in rats with different route of administration

目的研究静脉与左心室两种不同给药途径的腺苷预处理对在体缺血再灌注心肌的保护作用。方法48只成年SD大鼠随机分6组（n=8），分别为缺血再灌注组、缺血预处理组、腺苷静脉组、腺苷左心室组、生理盐水静脉组、生理盐水左心室组。建立大鼠在体缺血再灌注损伤模型，观察各组缺血再灌注前后心功能变化，并检测再灌注末血清肌钙蛋白T（cTnT）、丙二醛（MDA）、超氧化物歧化酶（SOD）的变化以及心肌组织核转录因子KB（NF—κB）的表达。结果缺血预处理组、腺苷静脉组及腺苷左心室组SOD值[（208．63±23．88）、（178．27±11．56）、（191．31±28．14）U／ml]均高于缺血再灌注组[（145．05±23．18）U／ml，P〈0．05]；cTnT、MDA值、NF—KB的表达均低于缺血再灌注组（P〈0．05）；心功能均好于缺血再灌注组。腺苷左心室组SOD值高于腺苷静脉组（P〈0．05）；cTnT、MDA值、NF—κB的表达低于腺苷静脉组（P〈0．05）。结论腺苷预处理可以模拟缺血预处理的心肌保护作用。左心室给药的腺苷预处理的心肌保护作用优于静脉给药的腺苷预处理的心肌保护作用。

Objective To evaluate the effect on myocardial protection of adenosine preconditioning in different route of administration through fight jugular vein and left ventricle. Methods Forty-eight SD rats were randomly divided into ischemia reperfusion group （blank control）, isehemic preconditioning group （IP, positive control）, adenosine venous infusion group, adenosine ventricular group, normal saline （NS） venous infusion group （negative control I） and NS ventricular group （negative control II）. The ischemia reperfusion rat models were established in vivo, and then changes of heart function, serum cardiac troponin T （cTnT）, superoxide dismutase （SOD）, malondialdehyde （MDA） and expression of nuclear factor- κB （NF- κB） were observed. Results SOD in IP [（208.63±23.88） U/roll ,adenosine venous infusion group[（178.27±11.56） U/ml] and adenosine ventricular group [（191.31 ±28.14） U/ml] were significantly higher than that in the ischemia reperfusion group [（145.05±23.18） U/ml] （P〈0.05）, cTnT, MDA and expression of NF-KB were lower than those in the ischemia reperfusion group （P〈0.05）. Heart function was significantly better than that in the ischemia reperfusion group （P〈0.05） ; SOD in adenosine ventricular group was significantly higher than that in adenosine venous infusion group （P〈0.05）. cTnT, MDA and expression of NF-κB were lower than those in adenosine venous infusion group （P〈0.05）. Conclusion Adenosine preconditioning may mimic protective effect of ischemic preconditioning. The effect on myocardial protection of adenosine in ventricular group is better than that of adenosine venous infusion group.