血液灌流对硫线磷和硫酸阿托品的吸附作用

Adsorption effect on cadusafos and atropine sulfate by hemoperfusion

目的定量研究血液灌流对有机磷农药硫线磷和其解毒药阿托品的吸附作用。方法模拟临床血液灌流装置，对含硫线磷和硫酸阿托品的血样进行灌流吸附，分别用毛细管气相色谱法和高效液相色谱法测定硫线磷和硫酸阿托品的残留量。结果吸附剂用量为0．5、1．0和1．5g，包膜活性炭在灌流2．0h后，硫线磷的清除率均能达到90％以上，硫酸阿托品的清除率依次为61．9％、84．9％和88．9％。HA230树脂在灌流1．5h后，硫线磷清除率都达到90％以上，硫酸阿托品的清除率也依次高达88．0％、97．2％和98．4％。包膜活性炭灌流3．0h后，硫酸阿托品与硫线磷的比值最高，为灌流前的10．1倍，而HA230树脂灌流后，此比值最高为灌流前的6．7倍。结论包膜活性炭和HA230吸附树脂血液灌流1．5-2．0h均能清除血中大部分硫线磷，而且均能增加血中硫酸阿托品和硫线磷浓度的比值。

Objective To study the adsorption effect on cadusafos and atropine sulfate by hemoperfusion. Methods Hemoperfusion was performed for sheep blood samples with cadusafos and atropine by imitated extracorporeal closed circulating perfusion apparatus. Residual cadusafos was determined by gas chromatography and residual atropine was determined by high performance liquid chromatography. Results Dose of adsorption agent was 0.5, 1.0 and 1.5 g, respectively. Two hours after hemoperfusion with membrane coated activated charcoal, clearance rate of eadusafos in 3 groups all exceeded 90%, and clearance rate of atropine sulfate was 61.9%, 84.9%, 88.9%, respectively. One and a half hours after hemoperfusion with HA230 absorption resin, clearance rate of eadusafos in 3 groups all exceeded 90%, and clearance rate of atropine sulfate was 88.0%, 97.2%, 98.4%, respectively. Three hours after hemoperfusion with membrane coated activated charcoal, the concentration ratio of cadusafos to atropine sulfate in blood was 10.1 times as compared to pre-hemoperfusion, and the ratio was 6.7 times after hemoperfusion with HA230 absorption resin. Conclusions Cadusafos is mostly removed from blood after 1.5 -2.0 hours hemoperfusion with membrane activated charcoal or HA230 absorption resin. The concentration ratio of cadusafos to atropine sulfate in blood increases after hemoperfusion.