应激因子对大鼠实验性脑梗死影响的研究

Effect of stress factor on focal cerebral ischemia in stress-induced rat

目的:探讨应激因子对大鼠实验性脑梗死的影响。方法:采用束缚加足底自动电脉冲刺激方法制成大鼠应激模型;采用MCAO大鼠局灶性脑缺血模型;通过检测大鼠束缚应激后血浆中应激因子—皮质醇(Cortisol)、白细胞介素-6(IL-6)、肿瘤坏死因子-a(TNF-a)、血小板活化因子(PAF)等应激标志物变化,探讨应激因子对大鼠实验性脑梗死的影响。结果:1应激24h内各时段血中Cortisol、IL-6、TNF-a和PAF的浓度变化显示,应激24h后均明显后增加(P<0.01),但短期1h段IL-6、TNF-a、PAF变化不明显。2应激1h后脑梗死范围与非应激脑梗死范围比较无显著差异(P>0.05),应激24h后脑梗死范围大于非应激脑梗死范围并有显著差异(P<0.01)。结论:短暂应激后应激因子只有Cortisol有一定程度增加,但对脑梗死范围影响不大;过度应激导致多种应激因子明显增加,综合因素加大脑梗死范围。

Objective： To study the effect of stress factor on focal cerebral ischemia in stress--induced rat. Methods：Restraint stress model was established by electric--foot companied restrain. After that the left middle common artery was occluded by MCAO. Stress factors （Cortisol, IL-6,TNF--a and PAF） were detected after restraint stress in order to study the effect of stress factor on focal cerebral ischemia in stress--induced rats. Re- sults：①The concentrations of Cortisol, IL--6, TNF--a and PAF increased significantly after 24 hours in stress -- group （P 〈 0. 01）, but the concentrations of IL--6,TNF--a and PAF did not significantly increase after 1 hour in stress--group. ②Cerebral infarction size after MCAO in stress 24h--group rats increased more than that of no--stress--group rats,with significant difference （P 〈 0.05）. Conclusion ：Only cortisol rises in some extent after transient stress, and however, cerebral infarction has not enlarged. Excessive stress leads to a marked increase in stressor and increases the size of cerebral infarction by combination factors.