白色念珠菌生物膜中胞外DNA分布观察

Distribution of the extracellular DNA in the formation of the candida albicans biomembrane

目的:动态观察白色念珠菌生物膜形成过程中胞外DNA的分布情况。方法:在2.0cm2载玻片上形成白色念珠菌生物膜,用核酸染料对膜中的胞外DNA进行染色,激光共聚焦显微镜下观察不同培养时间的生物膜中胞外DNA的分布。结果:白色念珠菌生物膜中含有胞外DNA。结论:白色念珠菌生物膜中含有胞外DNA,随培养时间延长,生物膜内胞外DNA的增加,分布在活菌的周围。

Objective：To make the dynamic observation of the extracellular DNA distribution in the formation of the candida albicans biomembrane, Methods： Biomembrane was formed on 2.0cm^2 poly- methylmcthacrylate glass slide. The distribution of the extraccllular DNA was observed by using confoeal laser scanning microscope （CLSM） in different incubation time. Result.The extracellular DNA was contained in the candida albicans biomembrane. Conclusion ：The extracellular DNA is contained in the candida albicans biomembrane; and the distribulion of the extracellular DNA becomes wider with culture time lasting .