环氧合酶-2在绝经后子宫内膜腺癌组织中表达及与病理学参数的相关性

Cyclooxygenase-2 Expression and Relation With Pathologic Parameters in Postmenopausal Endometrial Adenocareinoma.

目的探讨环氧合酶(cyclooxygenase,COX)-2在绝经后子宫内膜腺癌(endometrial adenocarcinoma)组织中的表达及与病理学参数的相关性,进而探讨其致癌作用。方法对2004年4月至2006年2月青岛大学医学院附属烟台毓璜顶医院收治的30例绝经后子宫内膜腺癌患者的子宫内膜组织标本(病例组)和10例绝经后子宫脱垂患者的子宫内膜组织标本(对照组)进行研究。采用免疫组织化学(immunohistochemistry,IHC)、原位杂交组织化学(in situ hybridization histochemistry,ISHH)及逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)法检测环氧合酶-2在30例绝经后子宫内膜腺癌组织及10例绝经后正常子宫内膜组织中的表达(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准)。结果①免疫组织化学法检测:环氧合酶-2蛋白在子宫内膜腺癌组织中的表达,显著高于正常子宫内膜组织(P<0.01);环氧合酶-2蛋白在高、中分化癌组织中的表达,显著低于低分化癌组织中的表达(P<0.01);但环氧合酶-2蛋白表达与子宫内膜腺癌病理学分期无关(P>0.05)。②逆转录酶聚合酶链反应检测:COX-2 mRNA在子宫内膜腺癌组织中的表达,显著高于正常子宫内膜组织(P<0.01);COX-2 mRNA在高分化子宫内膜腺癌中的表达,显著低于中、低分化癌组织(P<0.01),但与病理学分期无关(P>0.05)。③原位杂交组织化学法检测:子宫内膜腺癌组织中COX-2 mRNA的表达,显著高于正常子宫内膜组织(P<0.01);COX-2 mRNA表达随分化程度降低而升高(P<0.01),但与病理学分期无关。结论环氧合酶-2在绝经后子宫内膜腺癌的高表达,可能在该病的早期发生和发展中发挥重要作用。环氧合酶-2在绝经后子宫内膜腺癌组织中表达增高,可能参与肿瘤细胞浸润和转移。

Objective To investigate the expression and pathologic parameters of cyclooxygenase （COX）-2 in carcinogenesis of postmenopausal endometrial adenocarcinoma. Methods From April 2004 to February 2006, 30 patients with postmenopausal endometrial adenocarcinoma（EA group） and 10 patients with normal endometrial tissue（control group） were enrolled in this study in the Medical College of Qingdao University Affiliated Yantai Yu Huang Ding Hospital. Immunohistochemistry（IHC）, reverse transcriptase- polymerase chain reaction（RT-PCR） and in situ hybridization histochemistry（ISHH） methods were used to detect the expression of cyclooxygenase-2 in 30 cases of postmenopausal endometrial adenocareinoma tissues and 10 cases of normal endometrium tissues. Results ① Immunohistochemistry：Cyclooxygenase-2 protein expression was significantly higher in endometrial adenocarcinoma than that of corresponding normal tissue （P〈0.01）; cyclooxygenase-2 protein expression significantly decreased in gradeⅠ and Ⅱ than grade Ⅲ （P〈0.01）： cyclooxygenase-2 protein expression had no correlation to the pathological staging of endometrial adenocarcinoma （ P〉 0. 05 ）. ② Reverse transcriptase-polymerase chain reaction： Cyclooxygenase-2 mRNA expression was stronger in endometrial adenocarcinoma than that of corresponding normal tissue（P〈0.01）; cyclooxygenase - 2 mRNA expression was weaker in grade Ⅰ than grade Ⅱnd Ⅲ（P〈0.01） and nocorrelation to the pathological staging of endometrial adenocarcinoma. ③ In-situ hybridization histochemistry： Cyclooxygenase-2 mRNA expression was stronger in endometrial adenocarcinoma than that of corresponding normal tissue（P〈0.01）; cyclooxygenase-2 mRNA expression was stronger with grade increased（P〈0. 01） and no correlation with pathological staging. Conclusion Cyclooxygenase-2 shows high expression of postmenopausal endometrial adenocarcinoma. Cyclooxygenase-2 may be involved in the course of tumor angiogenesis of postmenopausal endometrial adenocarcinoma. Cyclooxygenase-2 overexpresses in postmenopansal endometrial adenocarcinoma, and might participate in the process of tumor invasion and metastasis.