差异表达基因分离技术的研究进展

Recent Advances on the Methods of Selecting Differentially Expressed Genes

分离并克隆差异表达基因是生命科学的研究热点。近年来,以差示筛选、扣除杂交等基本方法为基础,先后出现了抑制差减杂交,微阵列技术等多种分析差异表达基因的技术,使差异表达基因分离方法不断完善。对这些方法的优缺点、发展趋势及应用前景进行了简要综述。

Cloning differential expressed genes has become prime interest in molecular biology. In recent years, a variety of methods have been developed for analying differentially expressed genes, including differential display reverse transcription-PCR （ DDRT-PCR）, representational difference analysis （ RDA）, suppression subtractive hybridization （ SSH ） and cDNA microarray. This review described the principles, technical routes, improved methods, advantages and defects of these methods. Moreover, Associated applications and prospects in related studies were discussed.