摘要
利用青薯168、青薯5号和青薯8号三个品种幼芽带节茎段和节间组织为材料,研究了马铃薯的再生体系,结果表明,愈伤组织诱导率为72.92%～92.59%,愈伤组织分化率为30.68%～51.13%,每个外植体形成的小枝梢数为2.3～9.1个,绿色小植梢生根长成健壮的小植株,故认为MS+NAA0.25mg/L+BAP1.5mg/L+GA37mg/L+蔗糖30g/L+琼脂7g/L为愈伤组织诱导培养基,MS+BAP2.25mg/L+GA37mg/L+KT2.0mg/L+NAA2.25mg/L+蔗糖30g/L+琼脂7g/L为分化培养基组成的再生体系是有效的,特别对青薯8号更为明显。
The present article deals with in vitro induction callus and shoot regeneration of bud stem explants from three Qinghai cultivars： Qingshu168, Qingshu5 and QingshuS. The results shown that frequency of callus induction were 72.92-92.59%, frequency of differentiation were 30.68-51.13%, number of shoot per explant were 2.3-9.1. The suitable induction medium was MS ＋NAA0.25mg/L ＋ BAP1.5mg/L＋GA37mg/L＋ Sucrose 30g/L＋ agar 7g/L. The suitable differentiation medium was MS＋ BAP2.25mg/L＋ GA37mg/L＋KT2.0mg/L＋NAA2.25mg/L＋ Sucrose 30g/L＋agar7g/L.
基金
国家科技支撑-马铃薯育种(2006BAD01A06-1-10)
青海省重点科技攻关-马铃薯食品加工型型品种选育(2006-N-129)
