壳聚糖抗动脉粥样硬化的实验研究

Hyperlipoidemia and Anti-atherosclerosis Effects of Chitosan

目的探讨壳聚糖预防动脉粥样硬化形成的作用机制。方法将40只大鼠随机分为正常对照组,高脂模型组,壳聚糖高、低剂量组,各10只。实验至第9周末,麻醉后眼球取血,检测血清胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和丙二醛(MDA)、超氧化物歧化酶(SOD)活性。同时取主动脉作病理形态学观察。结果与高脂模型组比较,壳聚糖组大鼠血清TC、TG、MDA含量明显降低,HDL、SOD含量明显提高(P<0.05),主动脉壁厚度明显降低,泡沫细胞数量明显减少。结论壳聚糖具有减缓大鼠动脉粥样硬化形成的作用,其作用可能与降低TG、TC,提高HDL水平及抗脂质过氧化作用有关。

Objective To investigate the mechanism of anti-atherosclerosis effects by chitosan. Methods 40 rats were randomly divided into 4 groups ： normal control, hyperlipidemia model, chitosan at low dosage and one at high doasge. After 9 week＇s experiment, the total cholesterol （TC）, tryglyceride （TG）, low density lipoprotein （LDL）, high density lipoprotein（ HDL）, SOD and MDA in sera were detected, and pathomorphology of aorta was studied. Results Compared with those in the hyperlipidemia model rats,the levels of MDA and TC, TG in the sera of rats given with chitosan were lower, while the activity of SOD and level of HDL were increased. The aortic valves in rats fed with chitosan was significantly thinner and number of foam cells was less than those in the model rats. Conclusion The chitosan could attenuate production of atherogensis by lowing levels of TG,TC,MDA and elevating HDL and SOD.