乙型肝炎病毒表面抗原检测敏感度和标准化问题

Deficiencies in the Standardization and Sensitivity of Detection for Hepatitis B Virus Surface Antigen

乙型肝炎病毒（HBV）感染是一个全球性公共卫生问题。HBV表面抗原（HBsAg）是目前诊断急性和慢性HBV感染的最重要血清学标志物。但是HBsAg阴性并非意味着没有HBV感染，检测误差主要由病毒、宿主或者检测试剂盒造成。提高检测方法的敏感度有助于降低满检率，建立标准化检测可以发挥HBsAg定量检测在临床中的价值。同时结合HBVDNA和HBV核心抗体（HBcAb）可以提高HBV感染的检测率，保障临床输血和器官移植安全。

Hepatitis B virus （HBV） infection is a global public health problem. HBV surface antigen （HBsAg） is considered as a most important serum markers of diagnostic test for acute or chronic HBV infection. However,negative for HBsAg is not equal to being away from HBV infection. The reasons on deficiencies of detection are complex, such as virus itself, host or assay kit. It is important to elevate the sensitivity of detection in order to decrease the omission rate. Meanwhile, if we want to take advantage of the HBsAg quantitation in chnical, we should establish the standardized detection. In addition, HBsAg com- bined with HBV DNA and HBV core antibody, may increase the accuracy of detection on HBV infection, as well as ensure the safety of clinical transfusion and organ transplantation.