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TGF-β_1作用下JEG-3绒癌细胞Smad7蛋白表达的定量分析

QUANTITATIVE ANALYSIS OF SMAD7 EXPRESSION IN JEG-3 CHORIOCARCINOMA CELLS INDUCED BY TGF-β_1
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摘要 目的:研究转化生长因子β_1(TGF-β_1)作用下JEG-3绒癌细胞中Smad7蛋白的表达情况,探讨TGF-β/ Smads信号通路在绒癌中的功能作用。方法:分别用0ng/ml、100ng/ml及200ng/ml的TGF-β_1处理JEG-3绒癌细胞48h,采用蛋白印迹法定量检测JEG-3细胞Smad7的表达。结果:JEG-3绒癌细胞系可明确表达Smad7;不同浓度TGF-β_1作用下JEG-3细胞Smad7蛋白的表达无明显差别(P>0.05)。结论:绒癌细胞系JEG-3中Smad7对于外源性TGF-β_1的刺激无应答反应。 Objective：To study the effects of TGF- β1 on Smad7 expression in trophoblast cell line JEG-3, so to investigate the function of TGF-β/Smads signal transduction pathway in choriocarcinoma.Methods： Western blotting was used to detect the Smad7 expression in trophoblast cell line JEG-3 stimulated by TGF-β1 in different density.Results：Trophoblast cell line JEG-3 could express Smad7. The Smad7 expression in trophoblast cell line JEG-3 stimulated by TGF-β1 in different density had no obvious differences （P 〉 0.05）. Conclusions：Trophoblast cell line JEG-3 doesn＇t respond to stimulation of exogenous TGF-β1
作者 李晓茹 李玉红 许倩 张小红
机构地区 承德医学院
出处 《承德医学院学报》 2009年第2期117-119,共3页 Journal of Chengde Medical University
基金 河北省科技厅资助项目(07276101D-35)
关键词 TGF-Β1 SMAD7 蛋白印迹法 Choriocarcinoma TGF-β1 Smad7 Western blotting
分类号 R737.33 [医药卫生—肿瘤]
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参考文献11

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承德医学院学报
2009年 第2期

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