邻单胞菌L1对氯代苯的降解特性的研究

Degradation characteristics of chlorobenzene by Plesiomonas sp.L1 .

从某化工厂废水处理厂活性污泥中分离得到一株能以氯苯为唯一碳源的细菌，革兰氏阴性杆菌，兼性厌氧，初步鉴定为邻单胞菌属（Ｐｌｅｓｉｏｍｏｎａｓ）。该菌在有氧情况下１８天内对氯苯的降解率为５０％，在厌氧条件下１８天内对氯苯的降解率为３７．２％。不同底物试验结果表明，该菌还能利用间氯酚、ＴＣＰ、２，４Ｄ等二氯、三氯化合物，此外不经诱导抗汞能力达１５ｍｇ／Ｌ。细胞抽提物开环酶分析。

狝 bacterium which can degrade chlorobenzene as the sole source of carbon and energy was isolated by selective anaerobic enrichment from activated sludge.The bacterium was preliminarily identified as a Plesiomonas sp..The anaerobic and aerobic biodegradation rates of chlorobenzene determined by GC were 37.2% and 50%,respectively. The organism also grew on metachlorophenol, 1,2,4 trichlorobenzene, 2,4 dichlorophenoxyacetic acid. It had resistance to mercuric chloride with a concentration of 15mg/L without induction. Dioxygenase assays of cell extracts suggested that the strain L1 possessed an ortho pathway which was induced by chlorobenzene.