白屈菜红碱对肝纤维化大鼠肝脏病理学和肝脏羟脯氨酸含量的影响

Effects of chelerythine on hepatic pathology and hydroxyproline level in rats with CCl4-induced hepatic fibrosis

目的观察白屈菜红碱对四氯化碳诱导的肝纤维化大鼠肝脏病理学和肝脏羟脯氨酸(Hpy)含量的变化。方法采用四氯化碳联合营养控制和饮用10%酒精复合法制备SD大鼠肝纤维化模型,在实验第4周末,肝纤维化模型建立(2期)成功,然后应用不同剂量的白屈菜红碱和INF-γ处理。在给药8周后,处死所有大鼠,并取肝脏组织,采用HE、VG及Massion染色观察肝脏组织病理学形态的变化,同时采用改进的氯胺-T测定法检测肝脏Hyp含量。结果各剂量白屈菜红碱组肝纤维化半定量计分和肝脏的Hpy含量明显低于病理模型组(P<0.01);大中剂量白屈菜红碱组与小剂量组比,大鼠肝脏组织纤维化半定量计分及肝组织Hyp含量有明显下降,差异显著(P<0.01)。结论白屈菜红碱可以改善肝纤维化大鼠肝组织纤维化程度,降低肝组织Hyp含量,具有抗化学性肝纤维化作用。

Objective To observe the antifibrotie effects of ehelerythine in rats with CCl4-indueed hepatic fibrosis. Methods Hepatic fibrosis models in rats were established by injection of tetraehloride,in combination with the control of nutrition and the drinking 10% of alcohol. Four weeks later,ehelerythine and γ-interferon were used in two groups respectively,for comparison to control and fibrotie model group. At the end of eight weeks,the histological changes and hydroxyproline content were detected. Result The pathological fibrosis scores and liver hydroxyproline content in rats with ehelerythine or γ-interferon intervention decreased significantly as compared with fibrotic model group （P〈0.01）,and the effect of ehelerythine was found to be in a dose-dependent manner. Conclusions Chelerythine can improve the hepatic injuries in rats with tetraehloride-indueed hepatic fibrosis.