江西乙型肝炎病毒基因型分布及其与临床的相关性

Detection of hepatitis B viral genotypes in Jiangxi Province of China by nested PCR with multiplex pairs of primers

目的了解江西慢性乙型肝炎患者血清乙型肝炎病毒(HBV)基因型的分布情况及HBV基因型与临床的关系。方法选择HBV DNA阳性的慢性乙型肝炎患者血清150份,患者分别诊断为慢性HBV携带者、慢性肝炎轻度、中度、重度和慢性重型肝炎,每组30例,采用多对型特异性引物巢式PCR法检测HBV基因型。结果本组慢性乙型肝炎患者HBV基因分型结果为B型118例(78.7%),C型31例(20.7%),D型1例(0.6%);在上述5组患者中C型比例分别为0%,16.7%,26.7%,30.0%和30.0%,其中慢性HBV携带者与其他组比,差异有统计学意义(P<0.05);B、C两基因型患者HBeAg阳性率分别为83.9%(99/118)和90.3%(28/31),HBeAb阳性率分别为16.1%(19/118)和9.7%(3/31),差异均无统计学意义(P>0.05)。结论江西地区HBV优势基因型以B型为主,C型次之,有少量的D型感染者。

Objective To determine the hepatitis B virus （HBV） genotypes in Jiangxi Province by nested PCR with multiplex pairs of genotype-specific primers. Methods 150 serum samples from HBV DNA positive patients with chronic heptitis B virus infection were divided into 5 groups according to patient＇s diagnosis,with each group of 30 samples. The 5 groups were asymptomatic carrier,mild,moderate,severe hepatitis B and fulminant hepatitis B,respectively. Nested PCR with multiplex pairs of genotype-specific primers was used to determinate the genotypes of HBV. Results Of the 150 patients,118 （78.7%） were with genotype B,31 （20.7%） with genotype C and 1 （0.6%） with genotype D. The positive rates of HBV genotype C in patients with asymptomatic carrier,mild,moderate,severe hepatitis B and fulminant hepatitis B were 0%,16.7%,26.7%,30.0% and 30.0%,respectively,which was significantly different （P〈0.05） only between asymptomatic group and others. The HbeAg positivity in patients with genotype B and C infection were 83.9% （99/118）and 90.3%（28/31）,respectively and the HBeAb positivity were 16.1%（19/118） and 9.7%（3/31）,respectively （P〉 0.05）. Conclusion The predominant HBV genotypes in Jiangxi Province are confirmed to be genotypes B and C.