血管内皮生长因子与细胞支架复合物修复大鼠股骨缺损

Effect of vascular endothelial growth factor combined with cells-scaffold compound for the repair of rat femoral bone defects

目的:探讨血管内皮生长因子(VEGF)与骨髓间充质干细胞(MSCs)、纳米晶胶原基骨(nHAC)复合物对大鼠股骨缺损的修复作用。方法:将20只SD大鼠制成股骨缺损模型后分2组:对照组植入MSCs/nHAC复合物,实验组植入VEGF/MSCs/nHAC复合物。术后第2,4,8周行影像学和组织学观察;术后第8周行新生骨痂环境扫描电镜(ESEM)检查。结果:术后第2,4,8周实验组与对照组放射学检查评价骨生成差异有统计学意义(P<0.05)。组织学观察发现实验组较对照组能更快更有效地促进大鼠股骨缺损处的骨痂生长。结论:VEGF/MSCs/nHAC支架较MSCs/nHAC支架对骨缺损的修复有更好的效果。

Objective： To evaluate the effect of vascular endothelial growth factor（VEGF） combined with the compound of marrow mesenchymal stem cells （MSCs） and nano-hydroxyapatite/collagen（nHAC） bone on the treatment of rat femoral bone defects. Methods： Animal models of femoral bone defect were established in 20 Sprague-Dawley rats. They were divided into 2 groups randomly： In control group, defect was filled with nHAC/MSCs scaffolds; in experiment group was filled with MSCs/VEGF/nHAC. Imaging and histological observation were made in the 2nd,4th and 8th weeks after operation. Environment scanning electron microscope（ESEM） observation was made in the 8th week after operation. Results： There were significant differences in bone regeneration between control group and experiment group in the 2nd,4th and 8th weeks after operation （P 〈 0.05 ）. Histomorphology observation showed that new osteotylus of experiment group was earlier and more than control group. Conclusion： The bone defect repair ability of tissue-engineering bone which constructed with VEGF / MSCs/nHAC was stronger than MSCs/nHAC.