四川不同地区慈竹的遗传多样性研究

Assessment of genetic diversity in Neosinocalamus affinis from different regions of Sichuan province

【目的】比较四川不同地区慈竹的遗传多样性,为其遗传改良研究提供理论依据。【方法】以四川具有代表性的12个地区慈竹为材料,选取29条随机扩增多态性DNA标记（RAPD）和12条简单序列重复区间标记（IS-SR）引物,利用RAPD和ISSR技术,对慈竹的遗传多样性进行研究,并对扩增的特异性条带进行克隆分析。【结果】筛选出14个RAPD引物,扩增出168条条带,其中142条具有多态性,多态率高达84.52%;筛选出8个ISSR引物,扩增出73条条带,其中56条具有多态性,多态率为76.71%。慈竹RAPD和ISSR遗传距离分别为0.140 4-0.570 1和0.071 0-0.706 9。引物OPAA-04从眉山青神慈竹（C-MSQS）中可扩增出长度为570 bp的特异性条带,与细胞色素c氧化酶Ⅰ亚基相关。【结论】四川不同地区慈竹资源具有较高的遗传多样性。

【Objective】The study was done in order to provide a theoretical basis for genetic improvement by comparing the genetic diversity of the Neosinocalamus affinis from different regions in Sichuan province.【Method】Neosinocalamus affinis from the 12 typical regions of Sichuan was studied by using 29 randomly amplified polymorphic DNA（RAPD） and 12 inter-simple sequence repeat（ISSR） primers.The genetic diversity was studied by using RAPD and ISSR amplification markers and the specific band was cloned.【Result】14 RAPD primers were selected,168 bands amplified,and 142 bands polymorphic,of which the percentage of polymorphic bands（PPB） was 84.52% for RAPDs.8 ISSR primers were selected,73 bands amplified,and 56 bands polymorphic,of which the percentage of polymorphic bands（PPB） was 76.71%.The genetic distance ranged from 0.140 4 to 0.570 1 for RAPDs and 0.071 0 to 0.706 9 for ISSRs.The length of the special band of Neosinocalamus affinis from Qingshen county in Meishan city（C-MSQS） by primer OPAA-04 was 570 bp and the amplified fragment was related to cytochrome c oxidase subunit 1.【Conclusion】The Neosinocalamus affinis of Sichuan germplasm resources had a high genetic diversity.