HIF-1α、Caspase-3及Survivin在胸腺瘤中的表达及意义

Expression and significance of HIF-1α,Caspase-3 and survivin protein in thymoma

目的研究缺氧诱导因子-lα(HIF-1α)、Caspase-3及Survivin在胸腺瘤的发生、发展中的作用。方法应用免疫组织化学SP法检测60例上皮性胸腺肿瘤和30例非肿瘤胸腺组织中HIF-1α、Caspase-3、Survivin蛋白的表达。结果HIF-1α蛋白在胸腺瘤组织中的阳性率为61.67%(37/60),显著高于非肿瘤胸腺组织阳性率3.33%(1/30)(P<0.01);在侵袭性胸腺瘤中的阳性率为84.62%(33/39),显著高于非侵袭性胸腺瘤阳性率19.05%(4/21)(P<0.01);在恶性胸腺瘤中的阳性率为73.91%(34/46),显著高于良性(或低度恶性)胸腺瘤中的阳性率21.43%(3/14)(P<0.01)。Caspase-3蛋白在胸腺瘤组织中的阳性率为28.33%(17/60),显著低于非肿瘤胸腺组织阳性率96.67%(29/30)(P<0.01);在侵袭性胸腺瘤中的阳性率为5.13%(2/39),显著低于非侵袭性胸腺瘤阳性率71.43%(15/21)(P<0.01);在恶性胸腺瘤中的阳性率为10.87%(5/46),显著低于良性(或低度恶性)胸腺瘤中的阳性率85.71%(12/14)(P<0.01)。Survivin蛋白在胸腺瘤组织中的阳性率为73.33%(44/60),显著高于非肿瘤胸腺组织阳性率6.67%(2/30)(P<0.01);在侵袭性胸腺瘤中的阳性率为92.31%(36/39),显著高于非侵袭性胸腺瘤阳性率38.10%(8/21)(P<0.01)。在恶性胸腺瘤中的阳性率为84.78%(39/46),显著高于良性(或低度恶性)胸腺瘤中的阳性率35.71%(5/14)(P<0.01)。结论HIF-1α、Caspase-3及Survivin与胸腺瘤的发生有关;与胸腺瘤的恶性程度及侵袭性有关。检测HIF-1α、Caspase-3与Survivin可作为判断胸腺瘤生物学行为的客观指标。

Objective To explore the effects of HIF-1α,caspase-3 and survivin protein in thymoma occurrence. Methods The expressions of HIF-1 α, caspase-3 and survivin protein were detected in 60 cases of thymoma and 30 cases of non-neoplastic thymus tissue by immunohistochemical SP assay. Results 1. HIF-1α. The immunohistochemical staining showed that HIF-1α obviously expressed in thymoma. The positive rate was 61.67% （ 37/60）, significantly higher than non-neoplastic thymus tissue 3.33% （ 1/30 ） （P 〈 0. 01 ）. The positive rate in invasive thymoma （Masaoka-stage Ⅰ） was 84. 62% （33/39）, significantly higher than non-invasive thymoma（ Masaoka-stage Ⅱ-Ⅲ ）19. 05% （4/21） （P 〈 0. 01 ）. The positive rate in malignant thymoma（ WHO type B1 - B3 ） was 73. 91% （34/46）, significantly higher than in benign thymoma （ WHO type A, AB ） 21.43% （ 3/14 ） （ P 〈 0. 01 ） ; 2. Caspase-3 ： The positive rate of easpase-3 protein expressed in thymoma was 28. 33% （17/60）, significantly lower than in non-neoplastic thymus tissue 96. 67% （29/30） （P 〈 0. 01 ）. The positive rate in invasive thymoma was 5.13% （2/39）, significantly lower than in non-invasive thymoma 71.43% （ 15/21 ） （P 〈 0. 01 ）. The positive rate in malignant thymoma was 10. 87% （5/46）, significantly lower than in benign thymoma 85. 71% （ 12/14 ） （P 〈 0. 01 ） ;3. Survivin： The positive rate of survivin protein was 73.33 % （44/60） expressed in thymoma, significantly higher than in non-neoplastic thymus tissue 6. 67% （2/30） （P 〈0. 01 ）. The positive rate in invasive thymoma was 92. 31% （36/39）, significantly higher than in non-invasive thymoma 38. 10% （8/21） （P 〈 0. 01 ）. The positive rate in malignant thymoma was 84. 78% （39/46）, significantly higher than in benign thymoma 35.71% （5/14） （ P 〈 0. 01 ）. Conclusions HIF-1 α, easpase-3 and survivin were related to the genesis, malignant degree and invasive of thymoma. Detection of HIF-1α, caspase-3 and survivin could be used as the indicators to identify biological behavior of thymoma.