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慢病毒介导RNA干扰抑制脑胶质瘤细胞HMGA1表达对洛铂化疗敏感性的影响 被引量：2

Suppression of brain glioma cells expression of HMGA1 by lentivirus-mediated RNAi and its effect on lobaplatin chemosensitivity
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摘要 目的:建立逆转录病毒介导入高迁移率族蛋白组A1(HMGA1)基因RNA干扰体外表达体系,并观察洛铂对脑胶质瘤细胞株SHG-44化疗敏感性的影响。方法:制备HMGA1 siRNA慢病毒载体,PCR筛选阳性克隆,测序鉴定;转染脑胶质瘤细胞株SHG-44后,RT-PCR检测其对细胞HMGA1基因表达的影响;MTT和克隆集落实验检测洛铂对阳性组和阴性组细胞生长、增殖的影响。结果:PCR和测序证实,成功构建HMGA1 siRNA的慢病毒载体;RT-PCR证实阳性组细胞存在HMGA1基因表达下调;分别用不同浓度的洛铂处理两组细胞24h后,MTT法和克隆集落实验显示阳性组细胞生长抑制率较阴性组细胞明显降低。结论:HMGA1 siRNA能特异性下调脑胶质瘤细胞SHG-44中HMGA1的表达并抑制了其对洛铂的敏感性。 Objective： To investigate the lobaplatin chemosensitivity change of brain glioma cells of SHG-44 after high mobility group A1 （HMGA1） inhibition by lentivirus-mediated RNA interference （RNAi）. Methods： Lentiviral vectors of HMGAlsiRNA was constructed and verified by PCR and DNA sequencing; After transfected into the brain glioma cell line SHG-44 ,the expression of HMGA1 was determined by retrotranscriptase polymerase chain reaction（RT-PCR） ; The effect of lobaplatin on proliferation of positive and negative cells was observed by MTT assay and clonogenic survival assay. Results：PCR analysis and DNA sequencing demonstrated that the RNAi sequence targeting human HMGA1 gene was successfully inserted into the lentiviral vector; RT-PCR indicated that the expression of HMGA1 reduced obiviously in positive group. MTT assay and clonogenic survival assay showed that the inhibitory rate of positive group cells growth decreased significantly compared with negative group, treated with lobaplatin at different concentrations for 24h. Conclusion： RNAi vectors of HMGA1 could effectively suppress the expression of HMGA1 in SHG-44 cell line, and inhibit the sensitivity of brain glioma cells to lobaplatin.
作者 李帆 孙新臣 秦叔逵 成红艳 肖伟 张伟 孟庆红
机构地区 东南大学附属中大医院肿瘤科 解放军八一医院全军肿瘤中心
出处 《临床肿瘤学杂志》 CAS 2009年第5期405-409,共5页 Chinese Clinical Oncology
基金 南京市医学发展重点项目(ZKX0427) 国家“863”攻关计划重大专项(2006020608) 全军医学科学技术研究“十一五”计划(06MA111)
关键词 RNA干扰 高迁移率族蛋白组A1 洛铂 脑胶质瘤 RNA interference High mobility group A1 （HMGA1） Lobaplatin Brain glioma
分类号 R73-36 [医药卫生—肿瘤] R739.41 [医药卫生—肿瘤]
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参考文献13

  • 1Claus EB,Black PM.Survival rates and patterns of care for patients diagnosed with supratentorial low-grade gliomas[J].Cancer,2006,106(6):1358-1363.
  • 2Chang ZG,Yang LY,Wang W,et al.Determination of high mobility group A1(HMGA1) expression in hepatocellular carcinoma:a potential prognostic marker[J].Digestive Diseases and Sciences,2005,50(10):1764-1770.
  • 3Reeves R.Molecular biology of HMGA proteins:hubs of nuclear function[J].Gene,2001,277(1-2):63-81.
  • 4Chiappetta,G,Tallini G,De Biasio MC,et al.Detection of high mobility group I HMGI(Y) protein in the diagnosis of thyroid tumors:HMGI(Y) expression represents a potential diagnostic indicator of carcinoma[J].Cancer Res,1998,58:4193-4198.
  • 5Tamimi Y,van der Poel HG,Denyn MM,et al.Increased expression of high mobility group protein I(Y) in high grade prostate cancerdetemined by in situ hybridization[J].Cancer Res,1993,53:5512-5516.
  • 6Abe N,Watanabe T,Masaki T,et al.Pancreatic duct cell carcinomas express high levels of high mobility group I(Y) proteins[J].Cancer Res,2000,60:3117-3122.
  • 7Pierantoni GM,Agosti V,Fedel M,et al.High mobility group A1 proteins are overexpressed in human leukemias[J].Biochem J,2003,372:145-150.
  • 8Sarhadi VK,Wikman H,Salmenkivi K,et al.Increased expression of high mobility group A proteins in lung cancer[J].Pathol,2006,209(2):206-212.
  • 9Rho YS,Lim CY,Seok P,et al.High mobility group HMGI(Y) protein expression in head and neck squamous cell carcinoma[J].Acta Otolaryngo,2007,127(1):76-81.
  • 10Baldassarre G,Belletti B,Battista S,et al.HMGA1 protein expression sensitizes cells to cisplatin-induced cell death[J].Oncogene,2005,24:6809-6819.

二级参考文献13

  • 1Amir RE,Van den Veyver IB,Wan M,et al.Rett syndrome is caused by mutations in X-linked MeCP2,encoding methlyCpG-binding protein 2[J].Nat Genet,1999,23(2):185 -188.
  • 2Caballero IM,Hendrich B.MeCP2 in neurons:closing in on the causes of Rett syndrome[J].Hum Mol Genet,2005,14:R19 -R26.
  • 3Percy AK,Lane JB.Rett syndrome:clinical and molecular update[J].Curr Opin Pediatr,2004,16(6):670-677.
  • 4Shahbazian MD,Zoghbi HY.Rett syndrome and MeCP2:linking epigenetics and neuronal function[J].Am J Hum Genet,2002,71(6):1259-1272.
  • 5Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double stranded RNA in Caenorhabditis elegans[J].Nature,1998,391 (6669):806-811.
  • 6Mourrain P,Beclin C,Elmayan T,et al.Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance[J].Cell,2000,101 (5):533 -542.
  • 7Tabara H,Sarkissian M,Kelly WG,et al.The rde21 gene,RNA interference,and transposon silencing in C.elegans[J].Cell,1999,99(2):123-132.
  • 8Elbashir SM,Harborth J,Lendeckel W,et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells[J].Nature,2001,411 (6836):494-498.
  • 9Rubinson DA,Dillon CP,Kwiatkowski AV,et al.Alentivirus-based system to functionally silence genes in primary mammalian cells,stem cells and transgenic mice by RNA interference[J].Nat Genet,2003,33(3):401-406.
  • 10Naldini L,Blomer U,Gage FH,et al.Efficient transfer,integration,and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector[J].Proc Natl Acad Sci USA,1996,93:11382-11388.

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临床肿瘤学杂志
2009年 第5期

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