PPAR-γ配体对T淋巴瘤细胞NOS活性影响的研究

Study on NOS activities induced by PPAR-γ ligand in T cell lymphoma

目的:探讨人类Jurkat系T肿瘤细胞内,PPAR-γ对一氧化氮合酶(NOS)活性的影响及意义。方法:以Jurkat系T淋巴肿瘤细胞为研究对象,试验组采用PPAR-γ激活剂噻唑烷二酮类药物罗格列酮处理,分别在用药6h、12h、18h、24h和30h后用一氧化氮合酶测定试剂盒检测细胞裂解液NOS活性,RT-PCR法检测T肿瘤细胞PPAR-γ mRNA表达情况。结果:PPAR-γ mRNA表达量随用药时间的延长而升高,用药后在第6h、12h、18h、24h和30h的NOS活性均显著高于用药前NOS活性,差异有统计学意义(P<0.05),且NOS活性与PPAR-γ的表达呈正相关(r=0.905,P<0.01)。结论:在Jurkat系T肿瘤细胞内,PPAR-γ活化剂能够以时间依赖的形式增加NOS活性,PPAR-γ可能通过NOS途径对靶因子进行调节,发挥相应的抗瘤作用。

Objective：To investigate the influence and significance on nitric oxide synthase （ NOS ） production caused by peroxisome proliferator - activated receptor （ PPAR - γ） in human T tttmour cells. Mehods： External usage of the same concentration （20 μmol / L） of PPAR - γactivator rosiglitazone respectively on Jurkat T lymphatic cancer cells, NOS activities was detected by using nitric oxide enzymatic determination kit and PPAR - γ mRNA expression was detected by RT - PCR after treatment at 6h, 12h, 18h ,24h and 30h. Results ： The expression of PPAR -γ mRNA increased gradually with medication and NOS activities with administration of drug were significantly higher than the control group in the first 6h,12h,18h,24h and 30h,and the expressions of PPAR -γand NOS activities had positive correlation （ r = 0. 905, P 〈 0.01 ）. Conclusion： PPAR - γ activator was able to increase NOS activities relying on the time of drug usage and PPAR -γ might adjust the target factors by NOS pathway in Jurkat T tumour cells to play the appropriate anti - tumor role.