带状疱疹患者T细胞亚群及相关细胞因子的检测

Detection of T-lymphocyte Subsets and Correlated Cytokines in Herpes Zoster

目的了解T细胞免疫在带状疱疹发病中的作用,探讨带状疱疹发生的免疫学机制。方法用流式细胞仪技术和酶联免疫吸附法分别对病例组与对照组T细胞亚群表面标志CD3、CD4和CD8的表达情况,血清Th免疫相关因子白介素(IL)-2、IL-4和肿瘤坏死因子(TNF)-α水平进行分析。结果带状疱疹患者CD4比例显著降低,CD3比例亦降低,CD8比例升高,CD4/CD8比值下降;血清IL-2水平明显下降,TNF-α水平下降,IL-4水平明显升高。结论带状疱疹患者体内存在T细胞免疫功能异常,尤其CD4+T细胞的比例降低可能是疾病发生、发展的主要免疫学改变。

Objective To study the relationship between the expression of T cell and herpes zoster, and to investigate mechanism of pathogenesis of herpes zoster. Methods The expression of CD3, CD4 and CD8 on the T cell surface in patients and control group were determined by flow cytometry. The lever of IL-2, IL-4 and TNF-αin serum of patients and control group were determined by ELISA. Results During the disease process, CD3＋T cell and CD4T cell of herpes zoster patients decreased dramatically compared with that of control group, CD8＋T cell of patients increased dramatically compared with that of control group, CD4＋/CD8 ＋cell ratio reversed. The lever of IL-4 in herpes zoster patients serum increased dramatically compared with that in control group, and the lever of IL-2 and TNF-α decreased obviously. Conclusion The cell immune in herpes zoster patients became dysfunction, and CD4 ＋T cellular immunity may play a great role in the pathogenesis and development of herpes zoster.