嗜中性活性肽2(NAP-2)在人肝癌细胞株和肝癌组织的表达

Expressions of NAP-2 protein in human hepatoma cell line SMCC-7721 and hepatocellular carcinoma tissues

目的:探讨NAP-2在人肝癌细胞株SMCC-7721和人肝正常细胞株HL-7702、肝癌组织及其癌旁组织表达的差异性。方法:采用免疫细胞化学法(Immunocytochemistry,ICC)检测人肝癌细胞株SMCC-7721和人肝正常细胞株HL-7702中NAP-2蛋白的表达,免疫组织化学法(Immunohistochemistry,IHC)检测30例肝癌组织及其癌旁组织中NAP-2蛋白的表达。结果:NAP-2在人肝癌细胞株SMCC-7721表达率为100%,人肝正常细胞株HL-7702无表达。在肝癌组织中强阳性表达率为63.33%,癌旁组织无强阳性表达;经统计学分析,两者NAP-2的表达呈显著差异(P<0.01)。结论:NAP-2在肝癌发生、发展过程中可能起着一定作用。

Objective： To detect and analyze the expression of NAP - 2 in SMCC - 7721 and HL - 7702, hepatocellular carcinoma and its adjacent tissues. Methods：Immunocytochemical staining and immunohistochemical method were used to detect expressions of NAP -2 protein in SMCC -7721 and HL -7702,30 hepatocellular carcinoma tissues and their adjacent tissues ,respectively. Results：In SMCC -7721, the NAP -2 protein positive rates was 100%, there was no NAP - 2 protein expression in HL - 7702. In 30 hepatocellular carcinoma tissues, NAP - 2 protein strong positive rate was 63.33 % , but no strong expression in HCC adjacent tissues （P 〈 0.01 ）. Conclusion：NAP -2 protein may be related with the occurrence, development and progress of hepatocellular carcinoma.