口腔鳞癌与正常黏膜中T细胞分化蛋白mRNA的表达

Investigation on mRNA expression of T-cell differentiation protein in oral squamous cell carcinoma and paired normal tissues

目的:研究T细胞分化蛋白(T-cell differentiation protein,MAL)mRNA在口腔鳞状细胞癌(oral squamous cellcarcinoma,OSCC)及配对正常口腔黏膜组织中的表达及意义。方法:采用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔黏膜中MAL mRNA的表达,所得数据采用SAS6.12软件包进行χ2检验和t检验。结果:RT-PCR检测表明,MAL mRNA在30例OSCC和配对正常黏膜的阳性率分别为36.7%(11/30)和86.7%(26/30)(P<0.001);总表达水平分别为1.20±0.18和2.96±0.36,OSCC中表达水平较正常黏膜中表达下调2.47倍(P<0.001);MALmRNA在OSCC中表达下调与患者的性别、肿瘤的临床分期、有无颈淋巴结转移等临床参数无关,但与病理分化程度显著相关。结论:在OSCC的癌变过程中,MAL mRNA表达下调,表明MAL基因可能是OSCC的一个靶基因;MAL在OSCC诊断中具有潜在的应用价值。

PURPOSE： To investigate mRNA expression of T-cell differentiation protein（MAL） gene in oral squamous cell carcinoma（OSCC） and paired normal tissues. METHODS： Differential MAL gene expression in 30 cases of paired normal and cancerous tissues were detected by reverse transcription-PCR （RT-PCR）. The data were analyzed with SAS6.12 software package for Chi-square and t test. RESULTS： RT-PCR results demonstrated that the positive rate of MAL mRNA in 30 cancerous tissues and paired normal samples were 36.7% （11/30）and 40% 86.7% （26/30）, respectively （P〈0.001）; relative expression level of MAL mRNA in 30 cancerous tissues and paired normal samples were 1.20±0.18 and 2.96±0.36, respectively （P〈0.0001）, totally down-regulated 2.47-fold. Additionally,the expression of MAL mRNA was significantly associated with the grade of tumor differentiation. No significant difference of MAL mRNA level was found between sexes, clinical stages, and cervical lymph node metastasis. CONCLUSIONS： MAL mRNA overexpression may play a significant role in OSCC carcinogenesis and may be a potential molecular marker of OSCC.