应用免疫PCR法研究抗双链DNA抗体识别的DNA序列

STUDIES OF DEFINED OLIGONUCLEOTIDES RECOGNIZED BY ANTIDSDNA ANTIBODIES

用１段合成的１０８ｂｐＤＮＡ及免疫ＰＣＲ法，研究了ＳＬＥ血清抗双链ＤＮＡ抗体识别的ＤＮＡ序列。结果发现：１０个长度为１０８ｂｐ序列各异的已知ＤＮＡ片段，同具有亿万变化的１０８ｂｐＤＮＡ片段库一样，与抗双链ＤＮＡ抗体有良好反应，其中间２５个ｂｐ的变异不影响抗体与抗原反应；

he authors had studied the DNA sequences recognized by antidsDNA antibodies in SLE sera by using synthetic oligonucleotides and an immunePCR method. The results are as follows:antidsDNA antibodies demonstrate a specific reaction with 10 defined, 108 bp oligonucleotides as that with a degenerate pool, and the central 25 bp random sequences do not affect recognition by antidsDNA antibodies. SLE sera peferentially select sequences that tend to form nonbetaDNA structures.