SE-S抗原的发现及其组织化学与超微结构分布

DISCOVERY OF SES ANTIGEN AND ITS HISTOCHEMICAL AND ULTRASTRUCTURAL LOCALIZATION

通过肝脏酶解灌流和密度梯度离心法获得的肝窦内皮细胞作为免疫原，建立一株单克隆抗体ＳＥ－Ｓ，它特异性识别大鼠肝窦内皮细胞，而与其它类型的内皮细胞或非内皮细胞无交叉反应。免疫电镜及免疫印迹分析显示，此抗体所识别的抗原（ＳＥ－Ｓ抗原）位于肝窦内皮细胞的膜表面和胞饮小泡的内表面，分子量为４４～４８ｋＤ。以上结果说明ＳＥ－Ｓ抗原与肝窦内皮细胞的特殊功能密切相关。因此，对于研究不同病理、生理状态下的肝脏。

 monoclonal antibody, SES, which specifically reacted with rat hepatic sinusoidal endothelial cells(HSE), was established with collagenase perfusionseparated HSE as immunogen. No crossreactivity was observed with other types of endothelial cells or nonendothelial cells. By immunoelectron microscopy and immunoblot aalysis, the antigen recognized by MAb SES was localized to the membrane surface and inner surface of pinocytotic vesicles of HSE, molecular weight was 44～48 kD. The above evidence strongly suggested that SES antigen was related to the specific function of HSE. Therefore, SES may be a useful marker to study the role of HSE in various pathophysiological conditions of the liver.